PFOR | pyruvate oxidoreductase

381 €

AS07 275 | clonality: polyclonal | host: rabbit | reactivity:Chlamydomonas reinhardtii


12 st
Item No:
AS07 275

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product information
Background PFOR (pyruvate-ferredoxin oxidoreductase) is an enzyme which belongs to a family of oxidoreducatases and participates in 4 metabolic pathways: pyruvate metabolism, propanoate metabolism, butanoate metabolism and reductive carboxylate cycle (carbon dioxide fication). Alternative names: pyruvate oxidoreductase, pyruvate synthetase, pyruvic-ferredoxin oxidoreductase.

KLH-conjugated synthetic peptide conserved in Chlamydomonas reinhardtii PFOR protein A8JEH2

Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum
Format Lyophilized in PBS pH 7.4
Quantity 200 ĩg
Reconstitution For reconstitution add 200 ĩl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Related products

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AS10 1625 | Anti-FNR | ferredoxin-NADP+-oxidoreductase antibody

anti-rabbit secondary antibodies

Additional information
application information
Recommended dilution 1 : 10 000 (WB)
Expected | apparent MW

130 kDa

Confirmed reactivity Chlamydomonas reinhardtii
Predicted reactivity Volvox carteri
Not reactive in No confirmed exceptions from predicted reactivity are currently known.
Additional information
Selected references to be added when available, antibody released in April 2011

application example

Chlamydomonas reinhardtii total cell extract 15 µg/lane from cells acclimated to dark anoxia following  (1) 2 h; (2) 4h; (3) 8 h of anoxia imposition. Total proteins were extracted with 50 mM Tris buffer (pH 8.0) containing 10 mM EDTA and 2% SDS,  and were separated by SDS-PAGE using a 10% polyacrylamide gel  and then transferred 1h at RT to PVDF membranes. The membranes were blocked with a 5% milk in TBS-T for 2 h. Blot was incubated in the primary anti-PFOR antibody at a dilution of 1: 1 000 over night at 4°C with agitation in 3 % milk. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in  for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturers instructions. Exposure time was 5 seconds.

Courtesty Dr. Caludia Catalanotti, Carnegie Institution, USA

 western blot detection of PFOR in Chlamydomonas


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