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Bromelain

218 €

AS09 552 | clonality: polyclonal | host: rabbit | reactivity: Ananas comosus

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AS09 552

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product information
Background

Bromelain (EC=3.4.22.32) is a cysteine protease isolated from pineapple stem.

Immunogen

native bromelain isolated and purified from Ananas comosus

Host Rabbit
Clonality Polyclonal
Clone
Purity Purified IgG
Format Lyophilized in phosphate buffer saline
Quantity 10 mg
Reconstitution For reconstitution please add 1 ml of sterile destilled water.
Storage

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications western blot (WB), ELISA (ELISA),immunofluorescence (IF), immunohistochemistry (IHC)
Related products

AS09 553 | anti-bromelain (biotinylated)

AS09 554 | anti-bromelain (affinity purified)

AS09 546 | anti-PPI | papain (biotinylated)

AS09 547 | anti-PPI | papain (affinity purified)

Plant protein extraction buffer

Secondary antibodies

Additional information

antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA,immunoblotting and enzyme inhibition.

application information
Recommended dilution

1: 1000 - 1: 100 000 for techniques listed above

Expected | apparent MW

39 kDa

Confirmed reactivity

Ananas comosus

Predicted reactivity

Ananas comosus

Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information

The IgG (7S) fraction is prepared from the antiserum by ammonium sulphate precipitation and ion exchange chromatography.

Selected references

to be added when available



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