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2 | Photosynthesis Tool Kit - quantitation

849 €

AS04 051 | clonality: polyclonal | host: rabbit | reactivity: higher plants, algae, cyanobacteria

PRODUCT INFORMATION IN PDF

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Item No:
AS04 051

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product information
Background

This kit allows quantitation of major photosynthetic proteins: Rubisco, PsaC (PSI) and PsbA (PSII) as well as determination of PSI/PSII ratios using the western blot technique.

The kit contains following global antibodies, protein standards and secondary anribody:
Rabbit anti-RbcL (Form I and Form II). Product number: AS03 037
Rabbit anti-PsaC. Product number: AS10 939
Rabbit anti-PsbA Product number: AS05 084
Rubisco protein standard. Product number: AS01 017S
PsaC protein standard. Product number: AS04 042S
PsbA protein standard. Product number: AS01 016S
Goat anti-Rabbit IgG (H&L), HRP conj. (50 µl), Product number: AS09 602

Immunogen

KLH-conjugated syntetic peptides for respective antibodies

Host Rabbit. Secondary antibody: Goat.
Clonality Polyclonal
Clone
Purity
Format
Quantity 50 ĩl of respective antibody. 100 ĩl / tube of each protein standard. 50 ĩl of secondary antibody
Reconstitution
Storage

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications western blot (WB)
Related products

Plant and algal protein extraction buffer

Secondary antibodies

Additional information Product information - Primary antibodies:

Product nuumber:

Product name:

Reconstitution:

Recommended dilution:

AS03 037

Rabbit Anti-RbcL

Global antibody

For reconstitution see lable on respective tube.

1:5000-10 000 with standard ECL

AS10 939

Rabbit Anti-PsaC

Global antibody

For reconstitution see lable on respective tube.

1:1000 with standard ECL

AS05 084

Rabbit Anti-PsbA

Global antibody

For reconstitution see lable on respective tube.

1:10 000 with standard ECL


* All primary antibodies in this kit are raised in rabbits.


Product information - Protein standards:

Product number:

Product name:

Concentration:

Size:

Western Blot – Positive Control:

AS01 016S

PsbA *

0.25 pmol/ μl

41.5 kDa#

To generate a standard curve, 3 loads are suggested (0.5, 2 and 4 ul). For most applications a sample load of 0.2 ug of chlorophyll will give a PsbA signal in this range.

A 2 ul load is optimal for most chemiluminescent detection systems to use as a positive control.

AS01 017S

RbcL *

0.15 pmol/ μl

52.7 kDa

To generate a standard curve, 3 loads are suggested (0.5, 2 and 4 ul). For most applications a sample load of 0.2 ug of chlorophyll will give a RbcL signal in this range.

A 2 ul load is optimal for most chemiluminescent detection systems to use as a positive control.

AS04 042S

PsaC *

0.15 pmol/μl

11.5 kDa#

To generate a standard curve, 3 loads are suggested (0.5, 2 and 4 µl). For most applications a sample load of 0.2 µg of chlorophyll will give a PsaC signal in this range.

A 2 ul load is optimal for most chemiluminescent detection systems as a positive control.


*These proteins are larger than a respective native protein due to the addition of His-tag

* For reconstitution of standards see lable on respective tube.


Product information - Secondary antibody:

AS09 602- Goat anti-Rabbit IgG (H&L), HRP conjugated, 50 µl (2x25 µl)


Educational information about Quantitative western blot can be found here:  detailed method description, video tutorial

application information
Recommended dilution
Expected | apparent MW
Confirmed reactivity

higher plants, algae, cyanobacteria

Predicted reactivity
Not reactive in
Additional information Estimation of PSI to PSII ratio can be done using quantitative western blot technique using anti-PsaC (PSI) and PsbA (PSII) antibodies.
References: Brown et al. (2007). Resource dynamics during infection of Micromonas pusilla by virus MpV-SP1. Environmental Microbiology 9(11): 2720-2727. Brown et al. (2008). Flux capacities and acclimation costs in Trichodesmium from the Gulf of Mexico. Marine Biology 154 (3): 413-422.
Selected references

MacKenzie et al (2005). Large reallocations of carbon, nitrogen and photosynthetic reductant among phycobilisomes, photosystems and Rubisco during light acclimation in Synechococcus elongatus are constrained in cells under low environmental inorganic carbon. Arch of Microbiol. 183: 190 - 202.
Bouchard et al. (2006) UVB effects on the photosystem II-D1 protein of phytoplankton and natural phytoplankton communities. Photochem and Photobiol 82: 936-951.
Morash et al. (2007) Macromolecular dynamics of the photosynthetic system over a seasonal developmental progression in Spartina alterniflora. Can J. of Bot. 85: 476-483(8)



||| For other applications or usage on species other than stated above Agrisera offers a payment-after-testing option. To learn more about this or for any questions please use the LiveChat option or contact us at support@agrisera.com

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