Elip | Early light induced protein

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AS06 147A  |  clonality: polyclonal  |  host: rabbit  |  reactivity:  Ch. reinhardtii, P. sativum, Vitis sp.


27 st
Item No:
AS06 147A

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product information

Early light-induced proteins (ELIPs) are light stress-induced proteins related to the chlorophyll a/b binding protein family from higher plants and green algae located in the thylakoid membranes and involved in photosynthesis.


KLH-comjugated synthetic peptide derived from known plant ELIP sequnces including Pisum sativum P11432

Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum
Format Lyophilized in PBS pH 7.4
Quantity 100 ĩg
Reconstitution For reconstitution add 50 ĩl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Related products

AS03 036 | anti-Elip2 | early light inducible protein 2

collection of antibodies to proteins involved in photosynthesis

Plant protein extraction buffer

Secondary antibodies

Additional information
application information
Recommended dilution 1 : 500-1 : 1000 (WB)
Expected | apparent MW

20 kDa (Pisum sativum)

Confirmed reactivity Pisum sativum, Vitis sp, Chlamydomonas reinhardtii
Predicted reactivity Hordeum vulgare, Nicotiana tabacum, Oryza sativa, Pisum sativum, Vitis sp.
Not reactive in
Additional information

Min. 30 µg of total protein has to be loaded per lane.

Selected references

to be added when available, antibody released in March 2016. 

application example

western blot using anti-Elip Global antibodies

About 30 µg of total protein from Chlamydomonas reinhardtii, extracted with pre-cooled buffer (10% glycerol, 20 mM HEPES, 5 mM MgCl2, 2.5 mM EDTA, 10 mM KCl, 1 mM PMSF, 0,5% DTT; pH=7,4) and denatured with SDS/mercaptoethanol loading buffer at 95C for 5 min, were separated on 12% SDS-PAGE and blotted at 60V for 2,5h to nitrocellulose membrane using tank transfer. Blots were blocked with 0.2% Tween-20 in TBS (overnight, at 5OC) with agitation. Blot was incubated in the primary antibody at a dilution of 1:500 in TBS-T (0.05% Tween-20 in TBS) overnight at 5OC with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed 3 times for 10 min in TBS at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from goat) diluted 1:15 000 in TBS-T, for 2h at RT with agitation. The blot was washed as above and developed using *DAB/H2O2 mixture in TBS for approximately 5 min.
* DAB = 3,3'-Diaminobenzidine

Courtesy of Dr. Anna Aksmann, Department of Plant Physiology and Biotechnology, University of Gdańsk, Poland

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