PEPC | Phosphoenolpyruvate carboxylase

Immunoblot analyses of photosynthetic enzymes. Examples of immunoblot analysis for the photosynthetic proteins Rubisco (A), PEPC (B), NADP-ME (C), and PEP-CK (D) extracted from leaves of selected grass species grown at glacial (180 ?l l–1, G) or ambient (400 ?l l–1, A) [CO2].

Western blot analysis of RuBISCO LSU (A) and PEPC (C) of maize plants. Maize seedlings were pre-treated with 100 µM NaHS for 8 d and then grown in a nutrient solution containing 1 µM Fe(III)-EDTA or 50 µM Fe(III)-EDTA for 12 d. Relative expression level is shown as the ratio of RuBISCO LSU:?-actin (B) and PEPC:?-actin (D) using Quantity One software. Data are presented as means ± SE. Columns labelled with different letters indicate significant differences at P<0.05. –Fe, 1 µM Fe; –Fe+NaHS, seedlings were pre-treated with 100 µM NaHS and then treated with 1 µM Fe; +Fe, 50 µM Fe; +Fe+NaHS, seedlings were pre-treated with 100 µM NaHS and then treated with 50 µM Fe.

Immunoblot analysis of photosynthetic enzymes. Immunoblot analysis for the photosynthetic proteins Rubisco, PEPC, PEP-CK, and NADP-ME extracted from leaves of eight C4 grasses belonging to three biochemical subtypes in control (C) or shade (S) environments. Loaded volumes varied between 4 ?l and 15 ?l in order to normalize the protein content to a common leaf area. Because of the small gel size, a limited number of samples (8–9) was loaded on an individual gel. Finally, all immunoblots of the studied protein and species were arranged in a composite figure. For uniform visualization, gamma settings of individual images were adjusted. A protein ladder was used for individual immunoblots; for simplicity, band size is referred to numerically.

Expression patterns of OsImp?1a and OsImp?1b. (A) OsImp?1a and OsImp?1b are nucleocytoplasmic?localized proteins. Total protein, the nucleus?depleted fraction and nucleus?enriched fraction were loaded onto a sodium dodecylsulfate?polyacrylamide gel electrophoresis (SDS?PAGE) gel and subjected to immunoblot analysis. Histone H3 and phosphoenolpyruvate carboxylase (PEPC) were used as nuclear and cytosolic markers, respectively. T, total protein extracts; N, nucleus?enriched fraction; C, nucleus?depleted fraction. (B) Expression of OsImp?1a and OsImp?1b in different tissues. Tissues were collected at the booting stage from IR24. (C) Expression of OsImp?1a and OsImp?1b after infection with Xanthomonas oryzae pv. oryzae (Xoo) strain PXO99.