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SPS | Sucrose phosphate synthase, global (40 ĩg, HRP-conjugated)

406 €

AS03 035A-HRP |  clonality: polyclonal  |  host: rabbit  |  reactivity: [global antibody] for  di and monocots  |  cellular [compartment marker] of cytoplasm for photosynthetic tissues.

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AS03 035-HRP

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product information
Background

SPS (sucrose phosphate synthase, EC 2.4.1.14) is the key enzyme of carbon flux into sucrose fixation in plants. It catalyzes the synthesis of sucrose-phosphate from UDP-glucose and fructose-6-phosphate predominantly in the cytosol of sucrose-source leaf tissue.

Immunogen

KLH-conjugated synthetic peptide derived from conserved region within plant SPS protein sequences, including Arabidopsis thaliana isoforms 1F Q94BT0, 2F, 3F and 4F. Oryza sativa Q67WN8, Solanum tuberosum Q43845

Host Rabbit
Clonality Polyclonal
Clone
Purity Affinity purified serum
Format Liquid in PBS pH 7.4, conjugated to HRP
Quantity 40 µg
Reconstitution
Storage

Store at +4°C for 12-18 months. A preservative may be added for long time storage up to 2 years.

Tested applications Western Blot (WB)
Related products
AS06 185 | anti-SPS | Sucrose phosphate synthase (maize)
AS03 035A | anti-SPS | Sucrose phosphate synthase, global
AS03 035-ALP | anti-SPS | Sucrose phosphate synthase, global (40 µg, ALP-conjugated)
AS15 2995 | anti-SPSA1 | Sucrose phosphate synthase isoform A1, rabbit antibody
AS15 2996 | anti-SPSA2 | Sucrose phosphate synthase isoform A1, rabbit antibody
AS15 2997 | anti-SPSC | Sucrose phosphate synthase isoform C, rabbit antibody

Plant and algal protein extraction buffer
Additional information
application information
Recommended dilution

1: 1000 - 1: 5000 with standard ECL (WB)

Expected | apparent MW

120 | 120-130 kDa (fragments of 30/90 kDa may be detected)

Confirmed reactivity

Arabidopsis thaliana, Colobanthus quitensis Kunt Bartl, Hordeum vulgare, Lycopersicum esculentum, Lycopersicum penelli, Solanum tuberosum, Triticum aestivum, Pinus strobus, Zea mays

Predicted reactivity

Brassica napus, Citrus sinensis, Glycine max, Nicotiana tabacum, Oryza sativa, Physcomitrella patens, Populus balsamifera, Robinia pseudoacaci, Ricinus communis, Saccharum officinarum, Solanum lycopersicum, Theobroma cacao, Vicia faba, Vitis vinifera 

Not reactive in

No confirmed exceptions from predicted reactivity known in the moment

Additional information

Peptide used to elicit anti-SPS antibodies is perfectly conserved in all isoforms of SPS in plants.

Selected references


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