ANTIBODY SHOP

Services
Name:
Phone:
E-mail:
Message

SPS | Sucrose phosphate synthase, global

371 €

AS03 035A |  clonality: polyclonal  |  host: rabbit  |  reactivity: [global antibody] for  di and monocots  |  cellular [compartment marker] of cytoplasm for photosynthetic tissues.

PRODUCT INFORMATION IN PDF

Qty: 
Availability:
4 st
Delivery:
Shipping:
Item No:
AS03 035A

Info: Product suggestions Read reviews

 

product information
Background

SPS (sucrose phosphate synthase, EC 2.4.1.14) is the key enzyme of carbon flux into sucrose fixation in plants. It catalyzes the synthesis of sucrose-phosphate from UDP-glucose and fructose-6-phosphate predominantly in the cytosol of sucrose-source leaf tissue.

Immunogen

KLH-conjugated synthetic peptide derived from conserved region within plant SPS protein sequences, including Arabidopsis thaliana isoforms 1F Q94BT0, 2F, 3F and 4F. Oryza sativa Q67WN8, Solanum tuberosum Q43845

Host Rabbit
Clonality Polyclonal
Clone
Purity Affinity purified serum
Format Lyophilized in PBS pH 7.4
Quantity 200 µg
Reconstitution For reconstitution add 100 µl of sterile water
Storage

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications
Western Blot (WB)
Related products
AS06 185 | anti-SPS | Sucrose phosphate synthase (maize)
AS03 035-HRP | anti-SPS | Sucrose phosphate synthase, global (40 µg, HRP-conjugated)
AS03 035-ALP | anti-SPS | Sucrose phosphate synthase, global (40 µg, ALP-conjugated)
AS15 2995 | anti-SPSA1 | Sucrose phosphate synthase isoform A1, rabbit antibody
AS15 2996 | anti-SPSA2 | Sucrose phosphate synthase isoform A1, rabbit antibody
AS15 2997 | anti-SPSC | Sucrose phosphate synthase isoform C, rabbit antibody

Plant and algal protein extraction buffer
Additional information
application information
Recommended dilution

1: 1000 - 1: 5000 with standard ECL (WB)

Expected | apparent MW

120 | 120-130 kDa (fragments of 30/90 kDa may be detected)

Confirmed reactivity

Arabidopsis thaliana, Colobanthus quitensis Kunt Bartl, Hordeum vulgare, Lycopersicum esculentum, Lycopersicum penelli, Solanum tuberosum, Triticum aestivum, Pinus strobus, Zea mays

Predicted reactivity

Brassica napus, Citrus sinensis, Glycine max, Nicotiana tabacum, Oryza sativa, Physcomitrella patens, Populus balsamifera, Robinia pseudoacaci, Ricinus communis, Saccharum officinarum, Solanum lycopersicum, Theobroma cacao, Vicia faba, Vitis vinifera 

Not reactive in

No confirmed exceptions from predicted reactivity known in the moment

Additional information

Peptide used to elicit anti-SPS antibodies is perfectly conserved in all isoforms of SPS in plants.

Selected references
Zhang et al. (2014). Heterologous expression of AtPAP2 in transgenic potato influences carbon metabolism and tuber development. FEBS Lett. 2014 Aug 27. pii: S0014-5793(14)00621-8. doi: 10.1016/j.febslet.2014.08.019.

Application example

10 µg of total leaf protein from (1) A.thaliana,(3) Zea mays and (4) Hordeum vulgare extracted with PEB (AS08 300) as well as 10 µg cytosolic protein from (2) A.thaliana were separated on 4-12% NuPage (Invitrogen) LDS-PAGE and blotted 1.5h (30V) to nitrocellulose. Filters were blocked 1h with 2% low-fat milk powder in TBS-T (0.1% TWEEN 20) and probed with anti-SPS (AS03 035A, 1:2000, 1h) and secondary anti-rabbit (1:20000, 1 h) antibody (HRP conjugated, Abcam) in TBS-T containing 2% low fat milk powder.  Antibody incubations where followed by washings in TBS-T (15, +5, +5, +5 min). All steps were performed at RT with agitation. Signal was detected with standard ECL (GE Healthcare) using a Fuji LAS-3000 CCD (90s, high sensitivity).

 

||| For other applications or usage on species other than stated above Agrisera offers a payment-after-testing option. To learn more about this or for any questions please use the LiveChat option or contact us at support@agrisera.com