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SIP2 | Small basic intrinsic protein 2-1

454 €

AS09 496 | clonality: polyclonal | host: rabbit | reactivity: Arabidopsis thaliana

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AS09 496

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product information
Background

SIP protein functions as a water channel to facilitate the transport of water across cell membrane. It is mainly localized to ER.Alternative name: small basic intrinistic protein 2-1

Immunogen

KLH-conjugated synthetic peptide conserved in Arabidopsis thaliana SIP2;1 Q9M1K3, AT3G56950

Host Rabbit
Clonality Polyclonal
Clone
Purity Serum
Format Liquid
Quantity 100 ĩl
Reconstitution
Storage Store lyophilized/reconstituted at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Tested applications ELISA (ELISA), Western blot (WB)
Related products

AS09 495 | antiAtSIP1;1 | aquaporin SIP1-1

collection of antibodies to endomembrane system

Plant protein extraction buffer

Secondary antibodies

Additional information

0.1 % sodium azide is added as preservative. For antibody re-suspending information check the tube lable.

Antibodies will detect target protein in a few µg of a crude preparation loaded per well. If purified preparations of vacuolar and plasma membranes are used, one µg load per well should be sufficient.

Method for plant ER isolation is available here.

application information
Recommended dilution 1 : 8000 (ELISA), 1 : 1000 (WB)
Expected | apparent MW

25.9 kDa

Confirmed reactivity Arabidopsis thaliana
Predicted reactivity
Not reactive in No confirmed exceptions from predicted reactivity are currently known.
Additional information

Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.

Diluted antibody solution can be used 2 to 3 times within one month if it contains 0.1 % sodium azide as preservative and is stored at -20ºC to -80ºC.

Manufactured by Operon Biotechnologies.

Selected references Ishikawa et al. (2005). Novel type aquaporin SIPs are mainly localized to the ER membrane and show cell-specific expression in Arabidopsis thaliana. FEBS Lett. 579:5814-5820.

application example

 

 

crude membrane fraction/lane from Arabidopsis thaliana stems (1,3) and crude membrane fraction from yeast cells expressing SIP2;1 (2,4) were separated on 12 % SDS-PAGE and blotted 1h to PVDF membrane (40 min. at 10 V using BioRad semidry transfer). Filters were blocked 1h with 5 % low-fat milk powder in TBS-T (0.05% Triton X.100). Membranes were washed 5 times with TBS-T, each time in a fresh polystyrene box and probed with anti-AtSIP2;1 antibodies (AS09 496, 1:1000, 1h) and secondary anti-rabbit (1:2000, 1 h). All steps were performed in RT with agitation.

Peptide used to elicit anti-SIP2;1 antibodies have been incubated with antibodies - right panel.

 

western blot using anti-AtSIP2;1 antibodies

||| For other applications, usage on species other than stated above or any other questions, please use the LiveChat option or contact us at support@agrisera.com