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BiP | Lumenal-binding protein (40 ĩg, HRP-conjugated)

406 €

AS09 481-HRP | clonality: polyclonal | host: rabbit | reactivity: A. thaliana, B. napus, C. sativus, R. sativa L. Tokinashi-daikon, S. oleracea, S. lycopersicum, S. tuberosum, T. aestivum, Z. mays, O. europaea, P. abies, P. patens, Ch. reinhardtii

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AS09 481-HRP

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product information
Background

BiP2 (Binding immunoglobulin protein) is localized in endoplasmic reticulum lumen (ER) and plays a role in protein assembly inside ER.  BiP protein is abundant under all growth conditions but its synthesis can increase under conditions that lead to the accumulation of unfolded polypeptides in endoplasmic reticulum (ER).

Immunogen

KLH-conjugated synthetic peptide derived from Arabidopsis thaliana BiP proteins: BiP1 At5g28540  Q9LKR3, BiP2 At5g42020 F4K007 , BiP3 At1g09080  Q8H1B3

Host Rabbit
Clonality Polyclonal
Clone
Purity Affinity purified serum
Format Liquid, conjugated to HRP
Quantity 40 µg
Reconstitution
Storage

Store at +4°C for 12-18 months. A preservative may be added for long time storage up to 2 years.

Tested applications ELISA (ELISA), western blot (WB)
Related products

AS09 615 | BiP2 | lumenal-binding protein 2, goat antibody
AS09 614 | BiP2 | lumenal-binding protein 2, hen antibody
AS09 481PRE | BiP | lumenal-binding protein, pre-immune serum
antibodies to plant endomembrane system proteins

Plant protein extraction buffer

Secondary antibodies

Additional information

Method for plant ER isolation is available here.

application information
Recommended dilution

1: 8000 (ELISA), 1: 2000 with standard ECL (WB)

Expected | apparent MW

73.5 | 80 kDa

Confirmed reactivity dicots: Arabidopsis thaliana, Brassica napus, Cucumis sativus, Nicotiana benthamiana, Raphanus sativa L. Tokinashi-daikon, Olea europaea, Spinacia oleracea, Solanum lycopersicum, Solanum tuberosum, monocots: Triticum aestivum, Zeay mays, trees: Picea abies, moss: Physcomitrella patens, algae: Chlamydomonas reinhardtii
Predicted reactivity

Capsella rubella, Vitis vinifera, Ricinus comminus, Glycine max, Citrus clementina, Citrus sinsensis, Prunus persica, Arabis alpina, Isatis tincorina, Eucalyptus grandis, Capsicum annuum, Triticum aestivium. dicots including: Nicotiana tabacum, monocots: Hordeum vulgare, Oryza sativa, trees: Picea sitchensis, Populus trichocarpa.

Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information

Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel. This antibody has so far not worked in IP.

Selected references


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