GST class-phi | Glutathione S tranferase

454 €

AS09 479 | clonality: polyclonal | host: rabbit | reactivity: A. thaliana, L. formosa, S. superba


2 st
Item No:
AS09 479

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product information

Glutathione S-transferase PM24 (EC= is an enzyme which performs conjugation of reduced glutathione to a range of exogenous and endogenous hydrophobic compunds. Alternative name: 24 kDa auxin-binding protein, glutathione S-transferse PM24


KLH-conjugated synthetic peptide derived from Arabidopsis thaliana P46422, At4g02520

Host Rabbit
Clonality Polyclonal
Purity Serum
Quantity 100 ĩl

store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.

Tested applications ELISA (ELISA), western blot (WB)
Related products

antibodies to plant endomembrane proteins

Plant protein extraction buffer

Secondary antibodies

Additional information

0.1 % sodium azide is added as preservative. For antibody re-suspending information check the tube lable.

Antibodies will detect target protein in a few µg of a crude preparation loaded per well. If purified preparations of vacuolar and plasma membranes are used, one µg load per well should be sufficient.

application information
Recommended dilution

1: 8000 (ELISA), 1: 1000 with standard ECL (WB)

Expected | apparent MW

24 | 26 kDa

Confirmed reactivity

Arabidopsis thaliana, Liquidambar formosa, Schima superba

Predicted reactivity

dicots including: Brassica juncea

Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information

Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.

Diluted antibody solution can be used 2 to 3 times within one month if it contains 0.1 % sodium azide as preservative and is stored at -20ºC to -80ºC.

Manufacture by Operon Biotechnologies.

Selected references Datta and Chattopadhyay (2015). Changes in the proteome of pad2-1, a glutathione depleted Arabidopsis mutant, during Pseudomonas syringae infection.
Chen et al. (2013). Photosynthetic and antioxidant responses of Liquidambar formosana and Schima superba seedlings to sulfuric-rich and nitric-rich simulated acid rain. Plant Physiol & Biochem.

application example



1 µg and 10 µg of crude membrane fraction/lane from Arabidopsis thaliana were separated on 12 % SDS-PAGE and blotted 1h to PVDF membrane (40 min. at 10 V using BioRad semidry transfer). Filters were blocked 1h with 5 % low-fat milk powder in TBS-T (0.05% Triton X.100). Membranes were washed 5 times with TBS-T, each time in a fresh polystyrene box and probed with anti-GST antibodies (AS09 479, 1:1000, 1h) and secondary anti-rabbit (1:1000, 1 h). All steps were performed in RT with agitation.


western blot detection using anti-GST antibodies

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