GST class-phi | Glutathione S tranferase
AS09 479 | clonality: polyclonal | host: rabbit | reactivity: A. thaliana, L. formosa, S. superba
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1: 8000 (ELISA), 1: 1000 with standard ECL (WB)
|Expected | apparent MW||
24 | 26 kDa
|Confirmed reactivity||Arabidopsis thaliana, Liquidambar formosa, Schima superba
dicots including: Brassica juncea
|Not reactive in||
no confirmed exceptions from predicted reactivity known in the moment
Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.
Diluted antibody solution can be used 2 to 3 times within one month if it contains 0.1 % sodium azide as preservative and is stored at -20ºC to -80ºC.
Manufacture by Operon Biotechnologies.
|Selected references||Datta and Chattopadhyay (2015). Changes in the proteome of pad2-1, a glutathione depleted Arabidopsis mutant, during Pseudomonas syringae infection.
Chen et al. (2013). Photosynthetic and antioxidant responses of Liquidambar formosana and Schima superba seedlings to sulfuric-rich and nitric-rich simulated acid rain. Plant Physiol & Biochem.
1 µg and 10 µg of crude membrane fraction/lane from Arabidopsis thaliana were separated on 12 % SDS-PAGE and blotted 1h to PVDF membrane (40 min. at 10 V using BioRad semidry transfer). Filters were blocked 1h with 5 % low-fat milk powder in TBS-T (0.05% Triton X.100). Membranes were washed 5 times with TBS-T, each time in a fresh polystyrene box and probed with anti-GST antibodies (AS09 479, 1:1000, 1h) and secondary anti-rabbit (1:1000, 1 h). All steps were performed in RT with agitation.
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