GST class-phi | Glutathione S tranferase
AS09 479 | clonality: polyclonal | host: rabbit | reactivity: A. thaliana, L. formosa, S. superba
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|Recommended dilution||1 : 8000 (ELISA), 1 : 1000 (WB)|
|Expected | apparent MW||
24 | 26 kDa
|Confirmed reactivity||Arabidopsis thaliana, Liquidambar formosa, Schima superba
|Predicted reactivity||Arabidopsis lyRatha, Brassica juncea, Brassica rapa, Bruguiera gymnorhiza, Capsella rubella,Capsicum annuum, Capsicum chinense, Gossypium hirsutum, Hyoscyamus muticus, Nicotiana sylvestris, Noccaea caerulescens, Physcomitrella patens, Solanum commersonii, Solanum tuberosum, Vigna radiata var. radiata|
|Not reactive in||No confirmed exceptions from predicted reactivity are currently known.|
Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.
Diluted antibody solution can be used 2 to 3 times within one month if it contains 0.1 % sodium azide as preservative and is stored at -20ºC to -80ºC.
Manufacture by Operon Biotechnologies.
|Selected references||Datta and Chattopadhyay (2015). Changes in the proteome of pad2-1, a glutathione depleted Arabidopsis mutant, during Pseudomonas syringae infection.
Chen et al. (2013). Photosynthetic and antioxidant responses of Liquidambar formosana and Schima superba seedlings to sulfuric-rich and nitric-rich simulated acid rain. Plant Physiol & Biochem.
1 µg and 10 µg of crude membrane fraction/lane from Arabidopsis thaliana were separated on 12 % SDS-PAGE and blotted 1h to PVDF membrane (40 min. at 10 V using BioRad semidry transfer). Filters were blocked 1h with 5 % low-fat milk powder in TBS-T (0.05% Triton X.100). Membranes were washed 5 times with TBS-T, each time in a fresh polystyrene box and probed with anti-GST antibodies (AS09 479, 1:1000, 1h) and secondary anti-rabbit (1:1000, 1 h). All steps were performed in RT with agitation.
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