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HDEL | Endoplasmic reticulum retention signal (100 ĩg)

383 €

AS10 683  |  clonality: monoclonal  |  host: mouse  |  reactivity:Barnyard Grass (E. crus-galli), B. vulgaris, G. hirsutum, H. vulgare, Sorghum sp., V. radiata,  S. cerevisiaeD. melanogaster

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AS10 683

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product information
Background

HDEL (his-asp-glu-leu) is a C-terminal tertapeptide found in yeast and plants which allows sorting of resident soluble proteins in the lumen of the endoplasmic reticulum.

Immunogen

synthetic HDEL peptide corresponding to the C-terminus of yeast Bip

Host Mouse
Clonality Monoclonal
Clone IgG2B (clone 2E7)
Purity
Format Liquid
Quantity 100 ĩg
Reconstitution
Storage

store at -20°C for 1 year; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications western blot (WB), immunofluorescence (IF), immunogold (IG)
Related products

AS10 683-25 | HDEL | endoplasmic reticulum retention signal mouse antibody, 25 µg

AS13 2665 | KDEL | endoplasmic reticulum retention signal (50 µg), rabbit antibody

collection of antibodies for endomembrane proteins

Plant and algal protein extraction buffer

Secondary antibodies

Additional information

protein G purified IgG2B in PBS, pH 7.4 with 0.09 % sodium azide and 50 % glycerol at concentration 1 mg/ml

application information
Recommended dilution

1: 100 - 1. 1000  (WB), 1. 50- 1: 500 (IF)

Expected | apparent MW

78 kDa

Confirmed reactivity

Barnyard Grass (E. crus-galli), B. vulgaris, G. hirsutum, H. vulgare, Sorghum sp., V. radiata, S. cerevisiae, D. melanogaster

Predicted reactivity

not analyzed 

Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information

antibody in concentration 1 µg/ml was sufficient for detection of HDEL-containing proteins in 10 µg of yeast cell lysate by colorimetric western blot. Clone 2E7.
For western blot results,  immunofluorescence and immunogold images please referr to Napier et al. 1992

Selected references Luo et al.  (2006). GRP78/BiP is required for cell proliferation and protecting the inner cell mass from apoptosis during early mouse embryonic development. Mol Cell Biol. 26(15): 5688-5697.
Napier et al. (1992). Immunological evidence that plants use both HDEL and KDEL for targeting proteins to the endoplasmic reticulum. J Cell Sci. 102: 261-271.


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