RPL37 | Ribosomal protein L37 (cytoplasmic)

345 €

AS12 2115 | clonality: polyclonal | host: rabbit | reactivity:Arabidopsis thaliana, Chlamydomonas reinhardtii


23 st
Item No:
AS12 2115

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product information

RPL37 (Ribosomal protein L37) belongs to the ribosomal protein L37e family (cytosolic large ribosomal subunit) and binds to the 23S rRNA.


Recombinant, full length RPL37 of Chlamydomonas reinhardtii, A8IBG1, expressed in E.coli

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 ĩl
Reconstitution For reconstitution add 50 ĩl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
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Plant and algal protein extraction buffer

Secondary antibodies

Additional information
application information
Recommended dilution 1 : 10 000 (WB)
Expected | apparent MW

10.5 kDa

Confirmed reactivity Arabidopsis thaliana, Chlamydomonas reinhardtii
Predicted reactivity Glycine max, Solanum lycopersicum, Oryza sativa, Ostreococcus lucimarinus, Pinus sp., Ricinus communis, Micromonas sp., Volvox carteri
Not reactive in No confirmed exceptions from predicted reactivity are currently known.
Additional information
Selected references Couso et al. (2017). Autophagic flux is required for the synthesis of triacylglycerols and ribosomal protein turnover in Chlamydomonas. J Exp Bot. 2017 Oct 19. doi: 10.1093/jxb/erx372.
Ramundo et al. (2013). Repression of Essential Chloroplast Genes Reveals New Signaling Pathways and Regulatory Feedback Loops in Chlamydomonas. The Plant Cell.

application example

western blot using anti-RPL37 antibodies 

10 µg of total protein from Chlamydomonas reinhardtii extracted with standard lysis buffer (Tris-HCl pH 6.8 50mM, 10mM EDTA, 2% SDS, Sigma protease inhibitors)  were separated on 15 % SDS-PAGE and blotted to nitrocellulose membrane using a wet transfer cell. Blot was blocked in TBS-T containing 5% non-fat dry milk  for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 10 000 in TBS-T containing 5% non-fat dry milk  for 1h at RT with agitation. The antibody solution was decanted and the blot was washed  3 times for 15 min  in TBS-T containing 1% non-fat dry milk with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Promega) diluted to 1:0 000 in TBS-T containing 1% non-fat dry milk  for 1h at RT with agitation. The blot was washed as above  and developed for 1 min with ECL according to the manufacturers instructions. Exposure time was 30 seconds.

Courtesy of Dr. Silvia Ramundo, University of Geneva, Switzerland




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