PDR8 | ABC transporter G family member 36
AS09 471 | clonality: polyclonal | host: rabbit | reactivity: Arabidopsis thaliana
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1: 8000 (ELISA), 1: 1000 with standard ECL (WB)
|Expected | apparent MW||
165 |150 kDa (Arabidopsis thaliana)
|Not reactive in||
Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.
Diluted antibody solution can be used 2 to 3 times within one month if it contains 0.1 % sodium azide as preservative and is stored at -20ºC to -80ºC.
Protocol for preparation of plant plasma membranes can be found under "more information" tab. Additoinal 160 kDa cross-reacting band is detected when working with chloroplast fractions.
Manufactured by Operon Biotechnologies.
|Selected references||Campe at al. (2015). ABC transporter PEN3/PDR8/ABCG36 interacts with calmodulin that, like PEN3, is required for Arabidopsis nonhost resistance. New Phytol. 2015 Aug 28. doi: 10.1111/nph.13582.
Yoshihiro et al. (2006). Loss of AtPDR8, a plasma membrane ABC transporter of Arabidopsis thaliana, causes hypersensitive cell death upon pathogen infection. Plant Cell Physiol. 47:309-318.
5 µg of crude membrane fraction/lane from Arabidopsis thaliana (1) and the same sample incubated with a free peptide used to produce this antibody (2) were separated on 12 % SDS-PAGE and blotted 1h to PVDF membrane (40 min. at 10 V using BioRad semidry transfer). Filters were blocked 1h with 5 % low-fat milk powder in TBS-T (0.05% Triton X.100). Membranes were washed 5 times with TBS-T, each time in a fresh polystyrene box and probed with anti-AtPDR8 antibodies (AS09 471, 1:1000, 1h) and secondary anti-rabbit (1:2000, 1 h). All steps were performed in RT with agitation.
5 day old Arabidopsis thaliana seedlings were fixed with 2% PFA in PBS for 60 minutes under vacuum. Whole seedlings were processed as described in Sauer et al., 2006 using an Insitu Pro Intavis AG robot. Rabbit anti-PDR8 antibody was diluted in 1: 400 and sheep anti-rabbit Cy3 (Sigma) were diluted in 1:600)
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