PIP1;3 | Aquaporin, plasma membrane intrinistic protein 1-3
AS09 504 | clonality: polyclonal | host: rabbit | reactivity: Oryza sativa, Zea mays
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1: 8000 (ELISA), 1: 2000 with standard ECL (WB)
|Expected | apparent MW||
30.7 | 26-29 kDa (Oryza sativa)
Oryza sativa, Zea mays
monocots including: Hordeum vulgare, Stipa sp. Triticum aestivum,
|Not reactive in||
no confirmed exceptions from predicted reactivity known in the moment
Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.
Diluted antibody solution can be used 2 to 3 times within one month if it contains 0.1 % sodium azide as preservative and is stored at -20ºC to -80ºC.
Manufactured by Operon Biotechnologies.
Sakurai et al. (2008). Tissue and cell-specific localization of rice aquaporins and their water transport activities- Plant Cell Physiol. 49: 30-39.
1.5 µg of crude membrane fraction/lane from Oryza sativa L. Akitakomachi were separated on 12 % SDS-PAGE and blotted 1h to PVDF membrane (40 min. at 10 V using BioRad semidry transfer). Filters were blocked 1h with 5 % low-fat milk powder in TBS-T (0.05% Triton X.100). Membranes were washed 5 times with TBS-T, each time in a fresh polystyrene box and probed with anti-PIPs1;3 antibodies (AS09 504, 1:1000, 1h) and secondary anti-rabbit (1:2000, 1 h). All steps were performed in RT with agitation.
Below: Immunospecificity of anti.PIP1;3 antibodies to recombinant PIP proteins expressed in yeast cells. 1.5 µg protein/lane.
Most aquaporins show the dimerc forms in addition to monomer even in the presence of SDS.
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