PIP2;7 | Plasma membrane aquaporin (N-terminal)
AS09 469 | clonality: polyclonal | host: rabbit | reactivity: Arabidopsis thaliana, Jatropha curcas
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|Recommended dilution||1 : 8000 (ELISA), 1 : 2000 (WB)|
|Expected | apparent MW||
29.7 | 30.5 kDa (Arabidopsis thaliana)
|Confirmed reactivity||Arabidopsis thaliana, Jatropha curcas L. cv. "Biji Jarak "|
|Predicted reactivity||Brassica oleracea, Spinacia oleracea, Vitis vinifera, Juglans regia, Populus tremulax Populus tremuloides|
|Not reactive in||No confirmed exceptions from predicted reactivity are currently known.|
Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.
Diluted antibody solution can be used 2 to 3 times within one month if it contains 0.1 % sodium azide as preservative and is stored at -20ºC to -80ºC.
Theoretically this antibody can also react with PIP2;8 but it has not been confirmed experimentally.
Manufactured by Operon Biotechnologies.
|Selected references||Pou et al. (2016). Salinity-mediated transcriptional and post-translational regulation of the Arabidopsis aquaporin PIP2;7. Plant Mol Biol. 2016 Sep 26.
Hachez et al. (2014). The Arabidopsis Abiotic Stress-Induced TSPO-Related Protein Reduces Cell-Surface Expression of the Aquaporin PIP2;7 through Protein-Protein Interactions and Autophagic Degradation. Plant Cell. 2014 Dec;26(12):4974-90. doi: 10.1105/tpc.114.134080. Epub 2014 Dec 23.
Jang et al. (2013). Twoaquaporins of Jatropha are regulated differentially during drought stress and subsequent recovery. J Plant Physiol. March 25.
1 µg and 10 µg of crude membrane fraction/lane from Arabidopsis thaliana were separated on 12 % SDS-PAGE and blotted 1h to PVDF membrane (40 min. at 10 V using BioRad semidry transfer). Filters were blocked 1h with 5 % low-fat milk powder in TBS-T (0.05% Triton X.100). Membranes were washed 5 times with TBS-T, each time in a fresh polystyrene box and probed with anti-PIP2;7 antibodies (AS09 469, 1:1000, 1h) and secondary anti-rabbit (1:2000, 1 h). All steps were performed in RT with agitation.
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