PIP2;1, PIP2;2, PIP2;3 | Plasma membrane intrinistic protein 2-1,2-2,2-3
AS09 491 | clonality: polyclonal | host: rabbit | reactivity: A. thaliana, G. coquereliana, R. sativus
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1: 8000 (ELISA), 1: 1000 with standard ECL (WB)
|Expected | apparent MW||
30.4 | 28 (PIP2-1,PIP2-2,PIP2-3) kDa
Arabidopsis thaliana, Gromphadorhina coquereliana, Raphanus sativus
Camelina sativa, Brassica napus, Cucumis sativus, Glycine hispida, Gossypium hirsutum, Hedychium coronarium, Mimosa saman, Nicotiana glauca, Petunia hybrida, Pisum sativum, Ricinus communis, trees: Populus tremula x Populus tremloides, moss: Physcomitrella patens
|Not reactive in||
no confirmed exceptions from predicted reactivity known in the moment
Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.
Diluted antibody solution can be used 2 to 3 times within one month if it contains 0.1 % sodium azide as preservative and is stored at -20ºC to -80ºC.
Manufactured by Operon Biotechnologies.
|Selected references||Chowanski et al. (2015). Cold induced changes in lipid, protein and carbohydrate levels in the tropical insect Gromphadorhina coquereliana. Comp Biochem Physiol A Mol Integr Physiol. 2015 May;183:57-63. doi: 10.1016/j.cbpa.2015.01.007. Epub 2015 Jan 23.|
1 µg and 10 µg of crude membrane fraction/lane from Arabidopsis thaliana were separated on 12 % SDS-PAGE and blotted 1h to PVDF membrane (40 min. at 10 V using BioRad semidry transfer). Filters were blocked 1h with 5 % low-fat milk powder in TBS-T (0.05% Triton X.100). Membranes were washed 5 times with TBS-T, each time in a fresh polystyrene box and probed with anti-PIP2s antibodies (AS09 491, 1:1000, 1h) and secondary anti-rabbit (1:2000, 1 h). All steps were performed in RT with agitation.
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