PIP2;5 | aquaporin, plasma membrane intrinistic protein 2-5
AS09 508 | clonality: polyclonal | host: rabbit | reactivity: Oryza sativa
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|Recommended dilution||1 : 8000 (ELISA), 1 : 2000 (WB)|
|Expected | apparent MW||
29.7 | 26-29 kDa
|Confirmed reactivity||Oryza sativa|
|Predicted reactivity||Sorghum bicolor, Zea mays|
|Not reactive in||
Arabidopsis thaliana, Hordeum vulgare
Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.
Diluted antibody solution can be used 2 to 3 times within one month if it contains 0.1 % sodium azide as preservative and is stored at -20ºC to -80ºC.
Manufactured by Operon Biotechnologies.
|Selected references||Sakurai et al. (2008). Tissue and cell-specific localization of rice aquaporins and their water transport activities- Plant Cell Physiol. 49: 30-39.|
5 µg of crude membrane fraction/lane from Oryza sativa L. cv. Akitakomachi leaf blade and root were separated on 12 % SDS-PAGE and blotted 1h to PVDF membrane (40 min. at 10 V using BioRad semidry transfer). Filters were blocked 1h with 5 % low-fat milk powder in TBS-T (0.05% Triton X.100). Membranes were washed 5 times with TBS-T, each time in a fresh polystyrene box and probed with anti-PIP2;5 antibodies (AS09 508, 1:1000, 1h) and secondary anti-rabbit (1:2000, 1 h). All steps were performed in RT with agitation.
Peptide used to elicti this antibody has been added to primary antibody incubation (right panel, + +) and therefore specific signal has been depleted in this neutralization/immunocompetition assay.
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