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NRT1.4 | Nitrate transporter

454 €

AS09 473 | clonality: polyclonal | host: rabbit | reactivity: Arabidopsis thaliana, Raphanus sativus

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AS09 473

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product information
Background

Nitrate transporter functions in transporter activity in response to jasmonic acid stimulus and response to wounding. This protein is coded by ntp2 gene.

Immunogen

KLH-conjugated synthetic peptide derived from Arabidopsis thaliana nitrate transporter NRT1.4, Q9SZY4, At2g26690

Host Rabbit
Clonality Polyclonal
Clone
Purity Serum
Format
Quantity 100 ĩl
Reconstitution
Storage

store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.

Tested applications ELISA (ELISA), western blot (WB)
Related products

collection of antibodies to other proteins localized in tonoplast

Plant protein extraction buffer

Secondary antibodies

Additional information

0.1 % sodium azide is added as preservative. For antibody re-suspending information check the tube lable.

This protein is present in plant tissues in very low levels.

Protocol for isolation of plant vacuolar membranes can be found here.

application information
Recommended dilution

1: 8000 (ELISA), 1: 1000 with standard ECL (WB)

Expected | apparent MW

63.5 | 70 kDa (Arabidopsis thaliana)

Confirmed reactivity

Arabidopsis thaliana, Raphanus sativus

Predicted reactivity

Arabidopsis thaliana, Raphanus sativus

Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information

Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.

Diluted antibody solution can be used 2 to 3 times within one month if it contains 0.1 % sodium azide as preservative and is stored at -20ºC to -80ºC.

Manufactured by Operon Biotechnologies.

Selected references

to be added when available


application example

 

 

5 µg and 10 µg of vacuolar membrane fraction/lane from Raphanus sativus were separated on 12 % SDS-PAGE and blotted 1h to PVDF membrane (40 min. at 10 V using BioRad semidry transfer). Filters were blocked 1h with 5 % low-fat milk powder in TBS-T (0.05% Triton X.100). Membranes were washed 5 times with TBS-T, each time in a fresh polystyrene box and probed with anti-AJ011604 | nitrate transporter antibodies (AS09 473, 1:1000, 1h) and secondary anti-rabbit (1:2000, 1 h). All steps were performed in RT with agitation.

 

western blot using anti-nitrate transporter antibodies

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