MTP1 | vacuolar Zn2+/H+ antiporter
AS09 485 | clonality: polyclonal | host: rabbit | reactivity: Arabidopsis thaliana
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1: 8000 (ELISA), 1: 1000 with standard ECL (WB)
|Expected | apparent MW||
43.8 | 43 kDa (Arabidopsis thaliana)
dicots including: Brassica sp. , Noccaea fendleri, Thlaspi caerulescens,
|Not reactive in||
members of Solanaceae
Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.
Manufactured by Operon Biotechnologies.
Kawachi et al. (2008). Deletion of a histidine-rich loop of AtMTP1, a vacuolar Zn(2+)/H(+) antiporter of Arabidopsis thaliana, stimulates the transport activity. J.Biol. Chem. 13:8374-8383. Kobae et al. (2004). Zinc transporter of Arabidopsis thaliana AtMTP1 is localized to vacuolar membranes and implicated in zinc homeostasis. Plant Cell Physiol. 12:1749-1758.
sample of a vector (1) and vacuolar membrane fraction of yeast cells expressing AtMTP1 (2) separated on 12 % SDS-PAGE and blotted 1h to PVDF membrane (40 min. at 10 V using BioRad semidry transfer). Filters were blocked 1h with 5 % low-fat milk powder in TBS-T (0.05% Triton X.100). Membranes were washed 5 times with TBS-T, each time in a fresh polystyrene box and probed with anti-AtMTP1 antibodies (AS09 485, 1:1000, 1h) and secondary anti-rabbit (1:2000, 1 h). All steps were performed in RT with agitation.
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