TIP2 | Tonoplast intrinsic protein 2;1, 2;2, 2;3
AS12 2619 | clonality: polyclonal | host: rabbit | reactivity: Arabidopsis thaliana
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1: 1000 -1: 2000 with standard ECL (WB)
|Expected | apparent MW||
25.6 | 24 kDa (Arabidopsis thaliana)
|Confirmed reactivity||Arabidopsis thaliana|
|Predicted reactivity||Antirrhinum majus, Brassica oleracea, Chorispora exscapa, Gossypium mexicanum, Helianthus annuus, Medicago truncatula, Malus prunifolia, Nicotiana tabacum, Populus balsamifera subsp. trichocarpa, Raphanus sativus, Ricinus communis, Solanum nigrum , Solanum tuberosum, Vitis vinifera, Quercus petraea|
|Not reactive in||
no confirmed exceptions from predicted reactivity known in the moment
Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.
to be added when available, antibody releases in January 2014
10 µg of total proteinfrom Arabidopsis thaliana roots (Ecotype-Columbia) were separated on 12%SDS-PAGE and blotted 1h to PVDF membrane. Blots were blocked with 5%low-fat milk powderfor 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 over night with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T (0.05% Triton X.100)at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidaseconjugated from donkey diluted to 1:15 000 in for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL PlusTM (GE Healthcare Life Sciences, Amersham, UK) according to the manufacturer's instructions. Exposure time was 5 seconds.
Courtesy of Dr. Azeez Suliman Beebo, University of Gothenburg, Sweden
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