Ligand-gated ion channel 1.3
AS09 475 | clonality: polyclonal | host: rabbit | reactivity: Arabidopsis thaliana, Raphanus sativus
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1: 8000 (ELISA), 1: 2000 with standard ECL (WB)
|Expected | apparent MW||
97 |100 kDa
Arabidopsis thaliana, Raphanus sativus
to be determined
|Not reactive in||
no confirmed exceptions from predicted reactivity known in the moment
Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.
Diluted antibody solution can be used 2 to 3 times within one month if it contains 0.1 % sodium azide as preservative and is stored at -20ºC to -80ºC.
Manufactured by Operon Biotechnologies.
to be added when available
5 µg and 10 µg of vacuolar membrane fraction/lane from Raphanus sativus were separated on 12 % SDS-PAGE and blotted 1h to PVDF membrane (40 min. at 10 V using BioRad semidry transfer). Filters were blocked 1h with 5 % low-fat milk powder in TBS-T (0.05% Triton X.100). Membranes were washed 5 times with TBS-T, each time in a fresh polystyrene box and probed with anti-ligand-gated ion channel antibodies (AS09 475, 1:1000, 1h) and secondary anti-rabbit (1:2000, 1 h). All steps were performed in RT with agitation.
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