TIP1;1, TIP1;2 | tonoplast intrinistic protein 1-1, 1-2 (gamma)
AS09 493 | clonality: polyclonal | host: rabbit | reactivity: Arabidopsis thaliana, Raphanus sativus
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1: 8000 (ELISA), 1: 1000 with standard ECL (WB)
|Expected | apparent MW||
25.8| 23 kDa
|Confirmed reactivity||Arabidopsis thaliana, Raphanus sativus
dicots including: Brassica napus, Gossypium hirsutum, Ricinus communis, monocots including: Hordeum vulgare, Oryza sativa, trees: Populus trichocarpa,
|Not reactive in||
no confirmed exceptions from predicted reactivity known in the moment
Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.
Diluted antibody solution can be used 2 to 3 times within one month if it contains 0.1 % sodium azide as preservative and is stored at -20ºC to -80ºC.
|Selected references||Mao and Sun (2015). Arabidopsis seed-specific vacuolar aquaporins are involved in maintaining seed longevity under the control of ABSCISIC ACID INSENSITIVE 3. J Exp Bot. 2015 May 26. pii: erv244.
Suga et al. (2001). Specificity of the accumulation of mRNAs and proteins of the plasma membrane and tonoplast aquaporings in radish organs. Planta 212:294-304.
1 µg and 10 µg of crude membrane fraction/lane from Arabidopsis thaliana were separated on 12 % SDS-PAGE and blotted 1h to PVDF membrane (40 min. at 10 V using BioRad semidry transfer). Filters were blocked 1h with 5 % low-fat milk powder in TBS-T (0.05% Triton X.100). Membranes were washed 5 times with TBS-T, each time in a fresh polystyrene box and probed with anti-TIP1s antibodies (AS09 473, 1:1000, 1h) and secondary anti-rabbit (1:2000, 1 h). All steps were performed in RT with agitation.
TIP proteins usually show a faint band of dimeric form at 42 kDa in addition to the major monomer band of 23 kDa even in the presence of SDS.
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