V-ATPase, B | vacuolar H+-ATPase subunit B
AS14 2775 | clonality: polyclonal | host: rabbit | reactivity: Arabidopsis thaliana, Vigna radiata
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1: 1000 with ECL (WB)
|Expected | apparent MW||
53 | 57 kDa (Vigna radiata)
Arabidopsis halleri, Arabidopsis thaliana, Nicotiana tabaccum , Thellungiella salsuginea, Vigna radiata
Acetabularia sp. , Arundo donax, Chlamydomonas reinhardtii, Glycine soja, Gossypium mexicanum, Halostachys caspica, Haloxylon ammodendron, Hordeum vulgare, Medicago truncatula, Mesembryantheum crystallinum, Ostreococcus tauri, Oryza sativa, Panax ginseng, Physcomitrella patens, Pinus sylvestris, Populus trichocarpa, Pyrus sp., Ricinus communis, Theobroma cacao, Triticum aestivum, Zea mays, Zostera marina
|Not reactive in||
no confirmed exceptions from predicted reactivity known in the moment
Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.
|Selected references||to be added when available, antibody released in June 2015|
Proteins were separated by SDS-PAGE and transferred to an Immobilon-P membrane (Millipore) using Trans-Blot SD Semi-Dry Transfer Cell (Bio-Rad) with transfer buffer (100 mM Tris, 192 mM Glycine, 0.02% (w/v) SDS and 5% (v/v) methanol). After treatment with 1% ECL blocking agent (GE Healthcare), the membrane filter was incubated with the primary antibody (1:1000) and then with horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG (H+L) (Agrisera AS09 602, 1:25 000). Chemiluminescent reagent ECL (GE Healthcare) was used for detection of antigens. Chemiluminescence was detected with a Light-Capture II imaging device with a cooled CCD camera (Atto).
1: 10 μg of 100,000 x g precipitate prepared from Arabidopsis thaliana 6 weeks old shoot.
2: 0.2 μg of vacuolar membrane enriched fraction prepared from Arabidopsis thaliana 6 weeks old shoot.
3: 2 μg of vacuolar membrane enriched fraction prepared from Arabidopsis thaliana 6 weeks old shoot.
4: 0.2 μg of vacuolar membrane enriched fraction prepared from Vigna radiata 4 days old hypocotyls. 5: 2 μg of vacuolar membrane enriched fraction prepared from Vigna radiata 4 days old hypocotyls.
Courtesy of Drs. Masayoshi Maeshima and Dr Shoji Segami , Nagoya University, Japan
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