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Urease, Biotin conjugated

360 €

AS09 560 | clonality: polyclonal | host: rabbit | reactivity: Canavalia ensiformis (common jack-bean)

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AS09 560

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product information
Background

Urease (EC=3.5.1.5) is an enzyme involved in nitrogen metabolism. It is catalyzes the hydrolysis of urea into carbon dioxide and ammonia.

Immunogen

urease isolated and purified from Canavalia ensiformis (common jack-beans)

Host Rabbit
Clonality Polyclonal
Clone
Purity Purified IgG
Format Lyophilized in phosphate buffer saline
Quantity 1 ml
Reconstitution For reconstitution add 0.5 ml of sterile destilled water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications ELISA (ELISA), Western blot (WB), Immunofluorescence (IF), Immunohistochemistry (IHC)
Related products

AS09 559 | anti-urease

AS09 561 | anti-urease (affinity purified)

Plant protein extraction buffer

Additional information

Antibody is labelled with biotin using N-hydroxysuccinimidobiotin. Antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA,immunoblotting and enzyme inhibition.

application information
Recommended dilution 1 : 1000-1 : 30 000 (ELISA), (IF), (IHC), (WB)
Expected | apparent MW

91 kDa

Confirmed reactivity Canavalia ensiformis
Predicted reactivity Arabidopsis thaliana, Glycine max, Ricinus communis
Not reactive in No confirmed exceptions from predicted reactivity are currently known.
Additional information

Biotin/IgG protein molar ration is approximately 6.2. No foreign proteins are added.

Selected references

to be added when available



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