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Urease, Biotin conjugated

350 €

AS09 560 | clonality: polyclonal | host: rabbit | reactivity: Canavalia ensiformis (common jack-bean)

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AS09 560

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product information
Background

Urease (EC=3.5.1.5) is an enzyme involved in nitrogen metabolism. It is catalyzes the hydrolysis of urea into carbon dioxide and ammonia.

Immunogen

urease isolated and purified from Canavalia ensiformis (common jack-beans)

Host Rabbit
Clonality Polyclonal
Clone
Purity Purified IgG
Format Lyophilized in phosphate buffer saline
Quantity 1 ml
Reconstitution For reconstitution please add 1 ml of sterile destilled water.
Storage

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications western blot (WB), ELISA (ELISA),immunofluorescence (IF), immunohistochemistry (IHC)
Related products

AS09 559 | anti-urease

AS09 561 | anti-urease (affinity purified)

Plant protein extraction buffer

Additional information

Antibody is labelled with biotin using N-hydroxysuccinimidobiotin. Antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA,immunoblotting and enzyme inhibition.

application information
Recommended dilution

1: 1000 - 1: 30 000 for techniques listed above

Expected | apparent MW

91 kDa

Confirmed reactivity Canavalia ensiformis (common jack-bean)
Predicted reactivity

dicots including: Arabidopsis thaliana, Glycine max, Ricinus communis

Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information

Biotin/IgG protein molar ration is approximately 6.2. No foreign proteins are added.

Selected references

to be added when available



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