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Toc34 | chloroplast outer envelope membrane translocon complex protein

345 €

AS07 235  |  clonality: polyclonal  |  host: hen  |  reactivity: Pisum sativum

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Item No:
AS07 235

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product information
Background

Translocon at the outer envelope membrane of chloroplasts – Toc consists of three components: Toc34, Toc86, and Toc75. Toc 75 forms an outer membrane porin that may serve as the outer membrane proteinconducting channel.

Immunogen

soluble domain of Pisum sativum Toc34 overexpressed in E.coli  and purified by His-tag affinity purification. Overexpressed Toc34 is lacking the transmembrane C-terminal domain. 

Host Hen
Clonality Polyclonal
Clone
Purity Total IgY
Format Lyophilized in PBS pH 8.0 with 0.02% sodium azide
Quantity 50 ĩl
Reconstitution For reconstitution add 50 ĩl of sterile water.
Storage

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications western blot (WB), ELISA (ELISA), immunoprecipitation (IP)
Related products
AS07 211 | anti-Toc34 | OEP34, chloroplast outer envelope membrane translocon complex protein
 
AS07 237 | anti- Toc34 | OEP34, chloroplast outer envelope membrane translocon complex protein
 
AS07 238 | anti-Toc34 | OEP34, chloroplast outer envelope membrane translocon complex protein
Additional information

Pisum sativum Toc34 (chloroplast form) is Arabidopsis thaliana Toc33 that is expressed in the green leaves.

There is a slight MW shift between PsToc34 and PsToc33 which can be used to identify the dual expression however these two forms of the protein are differentially expressed.

application information
Recommended dilution

1 : 100 000 with alkaline phosphatase (WB)

Expected | apparent MW

29 kDa

Confirmed reactivity

Pisum sativum, Spinacia oleracea

Predicted reactivity

n.a.

Not reactive in

Arabidopsis thaliana

Additional information

to be added when available

Selected references Reddick et al. (2007). In vitro comparative kinetic analysis of the chloroplast Toc GTPases. J Biol Chem. 2007 Apr 13;282(15):11410-26.


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