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RbcS | Rubisco small subunit (SSU)

265 €
Buy 2 items of this product for 198 €/each
Buy 3 items of this product for 180 €/each

AS07 259 | clonality: polyclonal | host: rabbit | reactivity: A. thaliana, C. sativus, H. vulgare, M. domestica, N. tabacum, S. lycopersicum

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Item No:
AS07 259

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product information
Background

Rubisco catalyzes the rate-limiting step of CO2 fixation in photosynthesis. This enzyme contains two subunits, each present in eight copies. In plants and green algae, 55-kD large subunit is coded by the chloroplast rbcL gene, and the 15-kD small subunit is coded by a family of nuclear RbcS genes.

Immunogen

KLH-conjugated synthetic peptide derived from all known sequences of RbcS from monocots and dicots including RuBisCO small subunit 1B of Arabidopsis thaliana At5g38430

Host Rabbit
Clonality Polyclonal
Clone
Purity Serum
Format Lyophilized
Quantity 50 µl
Reconstitution For reconstitution add 50 µl of sterile water.
Storage

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications Western Blot (WB)
Related products AS07 259A | anti-RbcS | Rubisco small subunit (SSU) (affinity purified) rabbit antibodies
AS03 037 | anti-RbcL | Rubisco large subunit, form I and form II (50 µl), rabbit antibodies
AS03 037A | anti-RbcL | Rubisco large subunit, form I and form II (50 µg affinity purified), rabbit antibodies

AS03 037-HRP| anti-RbcL | Rubisco large subunit, form I and form II (40 µg, HRP-conjugated), rabbit antibodies

AS15 2955
| anti-RbcL II | Rubisco large subunit, form II (50 µl), rabbit antibodies
AS15 2955S | RbcL II | Rubisco form II positive control/quantitation standard

AS01 017  | anti-RbcL | Rubisco large subunit, form I, chicken antibodies
AS01 017S | Rubisco protein standard for quantitative western blot or positive control

AS03 037PRE | Rubisco large subunit, pre-immune serum
AS09 409 | Rubisco quantitation kit 
AS15 2994 | Rubisco ELISA quantitation kit 

AS07 218  | anti-Rubisco | 557 kDa hexadecamer, rabbit antibody to a whole protein

AS07 222 | anti-RbcS | Rubisco small subunit (SSU) from pea, rabbit antibodies

matching secondary antibody
Additional information
application information
Recommended dilution

1: 5000 with standard ECL (WB)

Expected | apparent MW

20 | 15 kDa

Confirmed reactivity

Arabidopsis thaliana, Cucumis sativus, Hordeum vulgare, Malus domestica, Nicotiana tabacum

Predicted reactivity

Camellia oleifera, Erythranthe guttata, Flaveria bidentis, Flaveria sonorensis, Glycine max, L, Nicotiana benthamiana, Oryza sativa, Petunia hybrida, Polianthes tuberosa, Populus deltoides, Triticum aestivum, Solanum melongena, Solanum tuberosum, Zea mays, liverwort Marchantia paleacea, green algae

Not reactive in

cyanobacteria

Additional information

This product can be sold containing ProClin if requested.

Selected references Yin et al. (2016). Interplay between mitogen-activated protein kinase and nitric oxide in brassinosteroid-induced pesticide metabolism in Solanum lycopersicum. J Hazard Mater. 2016 Oct 5;316:221-31. doi: 10.1016/j.jhazmat.2016.04.070. Epub 2016 Apr 29.
Robert et al. (2015). Leaf proteome rebalancing in Nicotiana benthamiana for upstream enrichment of a transiently expressed recombinant protein. Plant Biotechnol J. 2015 Aug 19. doi: 10.1111/pbi.12452.
Dahal et al. (2015). Improved photosynthetic performance during severe drought in Nicotiana tabacum overexpressing a nonenergy conserving respiratory electron sink. New Phytol. 2015 May 29. doi: 10.1111/nph.13479.
Krasuska et al. (2015). Switch from heterotrophy to autotrophy of apple cotyledons depends on NO signal. Planta. 2015 Jul 18.
Huang et al. (2015). Rubisco accumulation is important for the greening of the fln2-4 mutant in Arabidopsis. Volume 236, July 2015, Pages 185–194.
Kim et al. (2015). Cytosolic targeting factor AKR2A captures chloroplast outer membrane-localized client proteins at the ribosome during translation. Nat Commun. 2015 Apr 16;6:6843. doi: 10.1038/ncomms7843.
Sun et al. (2014). The response of rbcL, rbcS and rca genes in cucumber, (Cucumis sativus L.) to growth and induction light intensity. Acta Physiol Plant, October 2014, Volume 36, Issue 10, pp 2779-2791

||| For applications or usage on species others than stated above Agrisera offers a payment-after-testing option. To learn more about this or for any questions on this product, please use LiveChat option in the left menu or contact us at support@agrisera.com


||| For other applications, usage on species other than stated above or any other questions, please use the LiveChat option or contact us at support@agrisera.com