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AtpH | ATP synthase subunit c, chloroplastic

345 €

AS05 071  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: A. thaliana, S. oleracea, N. benthamina, T. elongatus

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Item No:
AS05 071

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product information
Background

F-type ATPase (ATP synthase) is the universal enzyme that synthesizes ATP from ADP and phosphate using the energy stored in a transmembrane ion gradient. Multiple copies of the c subunit build up the ring structure (in spinach a 14-mer of ~112 kDa) of the membrane bound Fo-part of the enzyme.

Immunogen

Purified c subunit from Spinacia oleracea UniProt: P69447.

Host Rabbit
Clonality Polyclonal
Clone
Purity Serum
Format Lyophilized
Quantity 200 ĩl
Reconstitution For reconstitution add 200 µl of sterile water.
Storage

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications Western Blot (WB)
Related products

AS08 370 | anti-ATP synthase whole enzyme

AS08 304 | anti-ATP synthase subunit alpha

AS05 085 | anti-ATP synthase subunit beta

AS08 312 | anti-ATP synthase subunit gamma antibody

Plant and algal protein extraction buffer

Secondary antibodies

Additional information

This product can be sold containing proclin if requested.

application information
Recommended dilution

1: 1000 - 1: 10 000 (WB)

Expected | apparent MW

8 kDa (for Arabidopsis thaliana)

Confirmed reactivity Arabidopsis thaliana, Nicotiana benthamina, Spinacia oleracea, Thermosynechococcus elongatus
Predicted reactivity

dicots including Glycine max, Pisum sativum, Vitis vinifera, monocolts indlucing Oryza sativaZea mays, trees: Populus alba, Pinus thunbergii, moss: Physcomitrella patens,  green algae

Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information

Note that increased incubation at 95ºC (20-30 min) prior to loading is recommended to break the multimeric c-mer structure, detection of partial ring structures (e.g. 5 or 6 subunits) may occur.

Selected references Nath et al. (2016). A Nitrogen-Fixing Subunit Essential for Accumulating 4Fe-4S-Containing Photosystem I Core Proteins. Plant Physiol. 2016 Dec;172(4):2459-2470. Epub 2016 Oct 26.
Lawrence et al. (2010). Recombinant production and purification of the subunit c of chloroplast ATP synthase. Protein Expression and Purification 76: 15-24.

application example

AS05 071 western picture.jpg

0.7 μg of   purified ATP-synthase complex from Spinacia oleracea (CF0F1) (1), 15.3 µg of purified ATP-synthase complex (CF0F1) from Nicotiana benthamiana (2) and 48.6 µg of Thermosynechococcus elongatus thylakoid preparation (3) were separated on 12% polyacrylamide gel and blotted on PVDF membrane. Filters where blocked (0.5h), incubated with 1: 1000 anti-AtpH antibodies (1h), followed by incubation with 1: 5 000 secondary anti-rabbit antibody (1.25h), coupled to HRP and visualized with standard ECL.


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