AtpG | ATPsynthase subunit II b', chloroplastic
AS09 457 | clonality:polyclonal | host:rabbit | reactivity: Arabidopsis thaliana, Chlamydomonas reinhardtii
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|Recommended dilution||1 : 10 000 (WB)|
|Expected | apparent MW||
22.4 kDa (Chlamydomonas reinhardtii) 15.9 kDa (Arabidopsis thaliana)
|Confirmed reactivity||Arabidopsis thaliana, Chlamydomonas reinhardtii|
Algae, Oryza sativa, Sorghum bicolor, Zea mays, Volvox carteri
|Not reactive in||No confirmed exceptions from predicted reactivity are currently known.|
Antibody reactivity for Arabidopsis thaliana has been confirmed on membrane fraction.
to be added when available. Antibodies released in November 2009.
10 ug of chlorophyll/well of Chlamydomonas reinhardtii total cell extract (1), Chlamydomonas reinhardtii subunit II deletion mutant thylakoid membrane fraction (2), Arabidospsis thaliana thylakoid membrane fraction (3), were separated on 12-18% acrylamide-8M urea gel and blotted to nitrocellulose membrane. Filters were blocked 1 h with 5% dry milk in 1 x PBS and probed with anti-ATP synthase subunit gamma antibody (AS09 457, 1: 10 000, 1h) and secondary HRP-conjugated anti-rabbit antibody (1: 10 000, 1 h) in 1 x PBS containing 5% dry milk. All steps were performed at RT with agitation. Signal was detected with standard ECL (GE Healthcare), exposure time was 1 min.
Arabidopsis membrane preparation has been done according to Lezhneva et al. (2008) A novel pathway of cytochrome c biogenesis is involved in the assembly of the cytochrome b6f complex in arabidopsis chloroplasts. J Biol. Chem., 283:24608-24616 and Chlamydomonas membranes were prepared according to Chua & Bennoun (1975) Thylakoid membrane polypeptides of Chlamydomonas reinhardtii: wild-type and mutant strains deficient in photosystem II reaction center. PNAS 72:2175-2179
Courtesy Dr. Yves Choquet
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