AtpG | ATPsynthase subunit II b', chloroplastic
AS09 457 | clonality:polyclonal | host:rabbit | reactivity: Arabidopsis thaliana, Chlamydomonas reinhardtii
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1: 10 000 (WB)
|Expected | apparent MW||
22.4 kDa (Chlamydomonas reinhardtii) 15.9 kDa (Arabidopsis thaliana)
Arabidopsis thaliana, Chlamydomonas reinhardtii
dicots including: Sorghum bicolor, monocots including: Oryza sativa, Zea mays, algae Volvox carteri
|Not reactive in||
no confirmed exceptions from predicted reactivity known in the moment
Antibody reactivity for Arabidopsis thaliana has been confirmed on membrane fraction.
to be added when available. Antibodies released in November 2009.
10 ug of chlorophyll/well of Chlamydomonas reinhardtii total cell extract (1), Chlamydomonas reinhardtii subunit II deletion mutant thylakoid membrane fraction (2), Arabidospsis thaliana thylakoid membrane fraction (3), were separated on 12-18% acrylamide-8M urea gel and blotted to nitrocellulose membrane. Filters were blocked 1 h with 5% dry milk in 1 x PBS and probed with anti-ATP synthase subunit gamma antibody (AS09 457, 1: 10 000, 1h) and secondary HRP-conjugated anti-rabbit antibody (1: 10 000, 1 h) in 1 x PBS containing 5% dry milk. All steps were performed at RT with agitation. Signal was detected with standard ECL (GE Healthcare), exposure time was 1 min.
Arabidopsis membrane preparation has been done according to Lezhneva et al. (2008) A novel pathway of cytochrome c biogenesis is involved in the assembly of the cytochrome b6f complex in arabidopsis chloroplasts. J Biol. Chem., 283:24608-24616 and Chlamydomonas membranes were prepared according to Chua & Bennoun (1975) Thylakoid membrane polypeptides of Chlamydomonas reinhardtii: wild-type and mutant strains deficient in photosystem II reaction center. PNAS 72:2175-2179
Courtesy Dr. Yves Choquet
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