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Cyt c6 | Thylakoid lumen cytchrome c6 protein

345 €

AS06 202 | clonality: polyclonal | host: rabbit | reactivity: Chlamydomonas reinhardtii | cellular [compartment marker] of thylakoid lumen

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Item No:
AS06 202

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product information
Background

Cytochrome c6 is known as a redox carrier of the thylakoid lumen of cyanobacteria and some eukaryotic algae that can substitute for plastocyanin in electron transfer.
Cytochrome c6 is situated between the two membrane-bound complexes cytochrome b6f and photosystem I (PSI) in oxygenic photosynthesis..
Cytochrome c6 (cyt c6) in Chlamydomonas reinhardtii is synthesized only under conditions of copper deficiency when plastocyanin cannot be synthesized.

Immunogen

GST fusion of recombinant Chlamydomonas reinhardtii cytochrome c6 A8J9G6

Host Rabbit
Clonality Polyclonal
Clone
Purity Serum
Format Lyophilized
Quantity 200 ĩl
Reconstitution For reconstitution add 200 ĩl of sterile water.
Storage

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications western blot (WB)
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AS05 084 | anti-PsbA rabbit antibody, marker of thylakoid membrane


Collection of antibodies to Chlamydomonas proteins

Additional information

cellular [compartment marker] of thylakoid lumen for Chlamydomonas reinhardtii

application information
Recommended dilution

1 : 1 000 with alkaline phosphatase (WB)

Expected | apparent MW

15 kDa

Confirmed reactivity

Chlamydomonas reinhardtii

Predicted reactivity

Chlorella vulgaris, Pediastrum duplex, Cladophora glomerata

Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information

Note: antibody will work better with nitrocellulose than with PVDF membrane. Recommended load per well min. 6 µg. Primary antibody incubation needs to be done over night. For detection image please refer to the reference below

Selected references Kropat et al. (2015). Copper economy in Chlamydomonas: Prioritized allocation and reallocation of copper to respiration vs. photosynthesis. Proc Natl Acad Sci U S A. 2015 Feb 2. pii: 201422492.
Merchant et al. (1998) Copper-Responsive Gene Expression in Photosynthetic Microorganisms. Methods in Enzymology 297:263-279.


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