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Cyt b6 / PetB | Thylakoid membrane cytochrome b6 protein, N terminal

345 €

AS03 034  |  clonality: polyclonal  |  host: rabbit  |  reactivity: A.thaliana, C.reinhardtii, F. bidentis, N. tabacum, T. weisflogii, T. pseudonana
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AS03 034

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product information
Background

Multi-subunit complex of cytb6/f is a crucial component for the photosynthetic electron transport chain of higher plants, green algae and cyanobacteria. This complex is catalyzing oxidation of quinols and the reduction the reduction of plastocyanin. This reaction allows to establish the proton force required for the ATP synthesis. Four major subunits build the complex: the petA gene product corresponding to a c-type cytochrome (cytf), the petB gene product corresponding to a b-type/c’-type cytochrome with three haems (cyt b6), the petD gene product (subunit IV, or suIV), and the petC gene product, corresponding to the Rieske/Iron/sulfur protein.

Immunogen

KLH-conjugated peptide chosen from N terminal sequence of cytb6 conserved in Arabidopsis thaliana P56773 and Chlamydomonas reinhardtii  NP_958365.1

Host Rabbit
Clonality Polyclonal
Clone
Purity Serum
Format Lyophilized
Quantity 100 µl
Reconstitution For reconstitution add 100 µl of sterile water.
Storage

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications Western Blot (WB)
Related products

AS08 330 | anti-PetC rabbit antibody

AS06 202 | anti-cytochrome c6

Collection of antibodies to Arabidopsis proteins

Collection of antibodies to Chlamydomonas proteins

Plant and algal protein extraction buffer

Secondary antibodies

Additional information
application information
Recommended dilution

1: 10 000 with standard ECL (WB)

Expected | apparent MW

23-25 kDa

Confirmed reactivity

Arabidopsis thaliana, Chlamydomonas reinhardtii, Flaveria bidentis, Nicotiana tabacum, Thalassiosira weisflogii, Thalassiosira pseudonana

Predicted reactivity

Hordeum vulgare, Pisum sativum, Oryza sativa, Populus alba, Salvia miltiorrhiza, Spinacia oleracea, Zea mays

Not reactive in

Synechocystis sp. 6803, Heterosigma akashiwo

Additional information

n.a.

Selected references Yoshida et al. (2016). Hisabori T1.Two distinct redox cascades cooperatively regulate chloroplast functions and sustain plant viability. Proc Natl Acad Sci U S A. 2016 Jul 5;113(27):E3967-76. doi: 10.1073/pnas.1604101113. Epub 2016 Jun 22.
Ishikawa et al. (2016). NDH-Mediated Cyclic Electron Flow Around Photosystem I is Crucial for C4 Photosynthesis. Plant Cell Physiol. 2016 Aug 6. pii: pcw127. [Epub ahead of print]
Yokono et al. (2015). A megacomplex composed of both photosystem reaction centres in higher plants. Nat Commun. 2015 Mar 26;6:6675. doi: 10.1038/ncomms7675.
Hojka et al. (2014). Inducible repression of nuclear-encoded subunits of the cytochrome b6f complex in tobacco reveals an extraordinarily long lifetime of the complex. Plant Physiol. 2014 Jun 24. pii: pp.114.243741.


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