Cyt f | Cytochrome f protein (PetA) of thylakoid Cyt b6/f-complex
AS06 119 | clonality: polyclonal | host: rabbit | reactivity: A. thaliana, C. reinhardtii, C. quitensis Kunt Bartl, C. pumilum, H. vulgare, N. gaditana, N. tabacum, P. patens, S. martensi, Synechocystis 6803 substrain PCC-M
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|Recommended dilution||1:2000 with alkaline phosphatase, 1: 50 000 with ECL (WB)|
|Expected | apparent MW||
34 | 31-32 kDa
|Confirmed reactivity||Arabidopsis thaliana, Chlamydomonas reinhardtii, Colobanthus quitensis Kunt Bartl, Craterostigma pumilum, Hordeum vulgare, Nannochloropsis gaditana, Nicotiana tabacum, Physcomitrella patens, Selaginella martensi, Synechocystis 6803 substrain PCC-M
|Predicted reactivity||Citrus x limon, green algae, Zea mays|
|Not reactive in||no confirmed exceptions from predicted reactivity known in the moment|
|Additional information||Reaction with cyanobacteria: Synechocystis 6803 and Synechococcus 7942 possible to obtain on total cell extract when using antibody at 1: 500 and longer exposure time.|
|Selected references||Georg et al. (2017). Acclimation of Oxygenic Photosynthesis to Iron Starvation Is Controlled by the sRNA IsaR1. Curr Biol. 2017 May 22;27(10):1425-1436.e7. doi: 10.1016/j.cub.2017.04.010. (Synechocystis PCC6803) Tyuereva et al. (2017). The absence of chlorophyll b affects lateral mobility of photosynthetic complexes and lipids in grana membranes of Arabidopsis and barley chlorina mutants. Photosynth Res. 2017 Apr 5. doi: 10.1007/s11120-017-0376-9. (Hordeum vulgare, western blot)
Ferroni et al. (2016). Light acclimation in the lycophyte Selaginella martensii depends on changes in the amount of photosystems and on the flexibility of the light-harvesting complex II antenna association with both photosystems. New Phytol. 2016 Apr 5. doi: 10.1111/nph.13939.
Suorsa et al. (2015). Light acclimation involves dynamic re-organisation of the pigment-protein megacomplexes in non-appressed thylakoid domains. Plant J. 2015 Aug 29. doi: 10.1111/tpj.13004.
Charuvi et al. (2015). Photoprotection Conferred by Changes in Photosynthetic Protein Levels and Organization during Dehydration of a Homoiochlorophyllous Resurrection Plant. Plant Physiol. 2015 Apr;167(4):1554-65. doi: 10.1104/pp.114.255794.
Hojka et al. (2014). Inducible repression of nuclear-encoded subunits of the cytochrome b6f complex in tobacco reveals an extraordinarily long lifetime of the complex. Plant Physiol. 2014 Jun 24. pii: pp.114.243741.
Dang et al. (2014). Combined Increases in Mitochondrial Cooperation and Oxygen Photoreduction Compensate for Deficiency in Cyclic Electron Flow in Chlamydomonas reinhardtii. Plant Cell. 2014 Jul 2. pii: tpc.114.126375.
Chlamydomonas reinhardtii samples are lysed cells, Arabidopsis thaliana samples are prepared from thylakoid membranes; Samples were boiled 1 min in 3%SDS,then 10ug of chlorophylls were loaded in each well of a 12% acrylamide gel.
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