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NdhS | NAD(P)H-quinone oxidoreductase subunit S (chloroplastic)

265 €
Buy 2 items of this product for 198 €/each
Buy 3 items of this product for 180 €/each

AS16 4066 | clonality: polyclonal | host: rabbit | reactivity: Arabidopsis thaliana

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Item No:
AS16 4066

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product information
Background

NAD(P)H-quinone oxidoreductase subunit 2 is involved in the transfer of electrons from NAD(P)H:plastoquinone, via FMN and iron-sulfur centers, to quinones in the photosynthetic chain and possibly in a chloroplast respiratory chain. Alternative names: NAD(P)H dehydrogenase, subunit 2, NADH-plastoquinone oxidoreductase subunit 2.

Immunogen

KLH-conjugated synthetic peptide dervied from Arabidopsis thaliana NdhS sequence, UniProt: A0A178V0R5, TAIR: AT4G23890

Host Rabbit
Clonality Polyclonal
Clone
Purity Affinity purified serum in PBS, pH 7.4
Format Lyophilized
Quantity 50 µg
Reconstitution For reconstitution add 50 ĩl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Related products

AS13 2711 | anti-NAD(P)H dehydrogenase subunit 5, rabbit antibodies

antibodies to proteins involved in electron transfer in chloroplasts

Secondary antibodies

Additional information
application information
Recommended dilution

1:10 000 (WB)

Expected | apparent MW

27 kDa

Confirmed reactivity Arabidopsis thaliana
Predicted reactivity

please inquire

Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information
Selected references

to be added when available, antibody released in October 2017.


application example

western blot using anti-NdhS antibodies 20 ug of precipites from Arabidopsis thaliana total leaf (extracted in a buffer containing 50 mM HEPES, 5 mM NaCl and 10 mM MgCl2) were separated in SDS-PAGE (15% acrylamide with 6M urea) and blotted 1h to a PVDF membrane using Höefer semi-dry blotter. Blot was blocked with 4 % milk in TTBS for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 10 000 overnight in +4°C. The antibody solution was decanted and the blot was washed 3 x 5 min with TTBS at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera, AS09 602) diluted to 1:20 000 in 1% milk/TTBS for 2h at RT with agitation. The blot was washed 3x5 min in TTBS and 1x5 min in TBS at RT with agitation and developed for 5 min with ECL according to the manufacturer's instructions. All samples apart from the ones marked "-AMS" are tagged with an alkylating agent AMS that binds free thiols and sligthly increases the MW of the protein, hence the mobility shift. "D" and "L" stand for dark and light adapted leaves. Courtesy Dr. Lauri Nikkanen, University of Turku, Finland

||| For other applications, usage on species other than stated above or any other questions, please use the LiveChat option or contact us at support@agrisera.com