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Pre-apoplastocyanin

345 €

AS06 118  | clonality: polyclonal  | host: rabbit  | reactivity: Chlamydomonas reinhardtii

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Item No:
AS06 118

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product information
Background

Plastocyanin is a “blue” copper protein which catalyzes electron transfer between the cytochrome b6 .f complex and P-700, the reaction center of photosystem I. Plastocyanin is a nuclear encoded polypeptide in all eukaryotic photosynthetic organisms where it has been studied. It is synthesized as a pre-protein of approximate molecular weight 17,000, imported post-translationally into chloroplasts, and processed to its mature form of approximate molecular weight 10,500 within the plastid.

Immunogen

GST fusion to Pre-apoplastocyanin of Chlamydomonas reinhartii  P18068

Host Rabbit
Clonality Polyclonal
Clone
Purity Serum
Format Lyophilized
Quantity 200 ĩl
Reconstitution For reconstitution add 200 ĩl of sterile water.
Storage

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications western blot (WB)
Related products

AS06 141 | anti-plastocyanin

Collection of antibodies to Chlamydomonas proteins

Algal protein extraction buffer

Secondary antibodies

Additional information
application information
Recommended dilution

1: 1000 with alkaline phosphatase (WB)

Expected | apparent MW

17 kDa (precursor)

Confirmed reactivity

Chlamydomonas reinhardtii

Predicted reactivity

Chlorella fusca Pediastrum boryanum

Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information

This antibody is recognizing pre-apoplatosyanin 17 kDa precursor and a mature protein. There is a slight cross-reactivity with cytochreom c6 in extract from Chlamydomonas reinhardtii cells grown under conditions of cooper deficiency.

Selected references

Li et al.  (1996).  Molecular genetic analysis of plastocyanin biosynthesis in Chlamydomonas reinhardtii.. J. Biol. Chem. 271:31283-31289



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