ANTIBODY SHOP

Services
Name:
Phone:
E-mail:
Message

HemC | Porphobilinogen-Deaminase (PBG)

345 €

AS08 375 | clonality:polyclonal | host:rabbit | reactivity:Thermosynechoccus elongatus

PRODUCT INFORMATION IN PDF

Qty: 
Availability:
17 st
Delivery:
Shipping:
Item No:
AS08 375

Info: Product suggestions Add review
product information
Background

PBG is an enzyme involved in tetrapolymerization of the monopyrrole PBG into the hydroxymethylbilane pre-uroporphyrinogen in several discrete steps.

Immunogen

overexpressed Thermosynechococcus elongatus HemC Q8DIE4

Host Rabbit
Clonality Polyclonal
Clone
Purity Serum
Format Lyophilized
Quantity 200 ĩl
Reconstitution For reconstitution add 200 ĩl of sterile water.
Storage

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications western blot (WB)
Related products

AS07 226 | anti-HemF

AS07 228 | anti-HemH | protoporphyrin ferrochelatase

Plant and algal protein extraction buffer

Secondary antibodies

Additional information
application information
Recommended dilution

1: 1000 - 1: 5000 (WB)

Expected | apparent MW

34.7 kDa

Confirmed reactivity

Thermosynechococcus elongatus

Predicted reactivity

cyanobacteria

Not reactive in

Synechococcus sp. PCC 6803

Additional information

to be added when available

Selected references

to be added when available


application example

0.5 ug chl from Synechocystissp. PCC 6803 membrane fraction (1) and soluble fraction (2) and Thermosynechococcus elongatus (3); Synechocystis was prepared as a soluble and membrane fractions. Separated on 12% SDS-PAGE and blotted overnight at 4C to PVDF membrane. Membrane was blocked for 1h using 0.2% tween 20 in TBS. Probed with anti-HemC antibody in TTBS (AS08 357, 1:2000, 4h) and secondary anti-rabbit in TTBS (1:10000,1h). Blot was developed for 5 min in West pico substrate and image of the blot was obtained using Fuji LAS 4000.

Courtesy Dr. Roman Sobotka Laboratory of Photosynthesis, Trebon

 

wesern blot detection using anti-PBG (HemC) antibodies 

||| For other applications, usage on species other than stated above or any other questions, please use the LiveChat option or contact us at support@agrisera.com