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HemH | Protoporphyrin ferrochelatase (cyanobacterial)

345 €

AS10 693  |  clonality: polyclonal  |  host: rabbit  |  reactivity:cyanobacteria

PRODUCT INFORMATION IN PDF

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AS10 693

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product information
Background

HemH is ptotoporphyrin ferrochelatase of heme biosynthesis.

Immunogen

KLH-conjugated peptide derived from cyanobacterial ferrochelatase protein sequence

Host Rabbit
Clonality Polyclonal
Clone
Purity Serum
Format Lyophilized
Quantity 100 ĩl
Reconstitution For reconstitution add 100 ĩl of sterile water
Storage

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications western blot (WB)
Related products

AS07 228 | HemH | protoporphyrin ferrochelatase (reacts with higher plants)

Algal protein extraction buffer

Secondary antibodies

Additional information
application information
Recommended dilution

1: 3000 with standard ECL, West pico (WB)

Expected | apparent MW

46 kDa

Confirmed reactivity

Synechocystis sp. PCC 6803

Predicted reactivity

cyanobacteria

Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information

loads higher than 0.5  µg of chlorophyll per well are not recommended

Selected references

to be added when available


application example

 

Membrane fraction (0.5 µg chl), prepared from Synechocystis sp. PCC 6803, was separated on 12% SDS-PAGE and blotted in 2 hours at RT to PVDF membrane. Membrane was blocked for 1h using 0.2% tween 20 in TBS, probed with anti-PsbI (1:10 000) antibody in TTBS overnight at 8ºC and secondary anti-rabbit in TTBS (1:10000,1h, room temperature). Blot was developed for 5 min in West pico substrate and image of the blot was obtained using Fuji LAS 4000.

Courtesy Dr. Roman Sobotka, Laboratory of Photosynthesis, Trebon,
Czech Republic




  western blot using anti-ferrochelatase antibodies (cyanobacterial)

 


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