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POR | Protochlorophilide oxidoreductase (10 ĩl)

149 €

AS05 067-10    clonality: polyclonal    host: rabbit  reactivity:  A.thaliana, H.vulgare, O. sativa, P.sativum, T.aestivum and cyanobacteria


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PRODUCT INFORMATION IN PDF

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AS05 067-10

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product information
Background

Chlorophylls are one of the most abundant classes of natural pigments and have an essential role in radiant energy absorptions during photosynthesis in bacteria, algae, and higher plants. Chlorophylls occur as noncovalently bound components of pigment-protein complexes, chloroplast-localized light-harvesting antennas LHC and photosynthetic reaction centers of PSI and PSII. Upon illumination the arrest in chlorophyll biosynthesis is ended and the etiolated parts of the plant and enzymatic photoreduction of protochlorophyllide (Pchlide) to chlorophyllide (Chlide), catalysed by POR enzyme.

Immunogen

native wheat POR protein isolated from a gel piece

Host Rabbit
Clonality Polyclonal
Clone
Purity Serum
Format Lyophilized
Quantity 10 ĩl
Reconstitution For reconstitution add 10 ĩl of sterile water.
Storage

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications western blot (WB)
Related products

AS05 067 | POR | protochlorophilide oxidoreductase (200ul)

AS08 300 | protein extraction buffer for plant and algal samples

Plant protein extraction buffer

Secondary antibodies

Additional information
application information
Recommended dilution

1:2 000 with standard ECL (WB)

Expected | apparent MW

36-37 kDa (Arabidopsis thaliana)

Confirmed reactivity

Arabidopsis thaliana, Horderum vulgare, Oryza sativa, Pisum sativum, Triticum aestivum and cyanobacteria

Predicted reactivity

n.a.

Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information

to be added when available

Selected references

Sakuraba et al. (2013). The rice faded green leaf locus encodes protochlorophyllide oxidoreductase B and is essential for chlorophyll synthesis under high light conditions. Plant J.

Yuan et al. (2012). Assembly of NADPH:protochlorophyllide oxidoreductase complex is needed for effective greening of barley seedlings. J. Plant Physiol. June 13.



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