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2 | Set of 4 Chlamydomonas anti-Lhc antibodies

1146 €

AS01 011 Chlamydomonas  |  clonality: polyclonal  |  host: rabbitreactivity: Chlamydomonas reinhardtii

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Item No:
AS01 011 CHLAMYDOMONAS

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This product includes following antibodies:

 

AS01 003  Lhcb2 | LHCII type II chlorophyll a/b-binding protein

AS06 117  Lhcb4 | CP29 (Lhcb4) homolog, Chlamydomonas

AS09 407  Lhcb5 | CP26 (Lhcb5) homolog, Chlamydomonas

AS09 408  Lhcbm5 | chlorophyll a-b binding protein of LHCII

product information
Background

The outer light-harvesting antenna of the photosystems (PSI and PSII) of the green unicellular alga Chlamydomonas reinhardtii is composed of pigment-binding proteins belonging to the Lhc family highly conserved in photosynthetic eukaryotes. Some of the Lhc-subtypes of Chlamydomonas share sufficient high similarity with the respective functional homologs in plants to allow specific detection with antisera generated against conserved peptide domains from plant Lhc-proteins.

Immunogen

Lhcb2 - BSA-conjugated peptide

Lhcb4 - KLH-conjugated synthetic peptide derived from Lhcb4 (CP29, Lhcbm4) protein sequence from Chlamydomonas reinhardtii (Q93WD2)

Lhcb5 - KLH-conjugated synthetic peptide derived from Chlamydomonas reinhardtii Lhcb5 protein sequence (Q9FEK6)

Lhcbm5 - SDS-PAGE purified polypeptide from Chlamydomonas reinhardtii LHCII-type II-enriched fractions

Host Rabbit
Clonality Polyclonal
Clone
Purity Total IgG in PBS pH 7.4 (Lhcb2), serum (Lhcb4, Lhcb5, Lhcbm5)
Format Lyophilized
Quantity 50 ĩl (Lhcb5, Lhcbm5), 100 ĩl (Lhcb4), 200 ĩl (Lhcb2)
Reconstitution
Storage

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications western blot (WB)
Related products

other antibodies against pigment-binding proteins LHC

Plant protein extraction buffer

Secondary antibodies

Additional information
application information
Recommended dilution

1:5000 with standard ECL (WB) (Lhcb2,Lhcb5, Lhcbm5)

1: 10 000 with standard ECL (Lhcb4)

Expected | apparent MW

25 kDa (Lhcb2)

29 kDa (Lhcb4)

29 | 30 kDa (Lhcbm5)

30| 26 kDa (Lhcb5)

Confirmed reactivity Chlamydomonas reinhardtii
Predicted reactivity

angiosperms (monocots and dicots), gymnosperms, mosses, green algae (Lhcb2)

Not reactive in

Lhcb4 - higher plants (use AS04 045 for those organsims), algae, cyanobacteria

Additional information

to be added when available

Selected references Lhcb5 and Lhcbm5 -
Takahashi et al. (2006). Identification of the mobile light-harvesting complex II polypeptides for state transitions in Chlamydomonas reinhardtii. PNAS 103:477-482


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