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2 | Set of 10 plant anti-Lhca and anti-Lhcb antibodies

1698 €

AS01 011  |  clonality: polyclonal  |  host: rabbit  |  reactivity: angiosperms (monocots and dicots) and gymnosperms

PRODUCT INFORMATION IN PDF

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AS01 011

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product information
Background

In plants the outer light-harvesting antenna of the photosystems (PSI and PSII) is composed of pigment-binding proteins belonging to the Lhc family. Of the 10 main conserved subtypes Lhca1-4 are exclusively associated with PSI while Lhcb3-6 are only found with PSII. Lhcb1 and Lhcb2 are found in the mobile trimers of LHCII associating with both photosystems in variable ratios (state1-state2 transitions). All Lhc polypeptides bind an individual set of chlorophyll a, chlorophyll b, and xanthophyll molecules in a structural and dynamic mutual arrangement which ensures high efficiency of energy transfer processes involved in light-harvesting and photoprotection.

Immunogen

BSA or KLH-conjugated synthetic peptides derived from conserved regions of plant Lhca1-4 and Lhcb1-6 protein sequences.

Host Rabbit
Clonality Polyclonal
Clone
Purity
Total IgG in PBS pH 7.4, affinity purified serum (Lhcb1) or serum (Lhcb5, and Lhcb6)
Format Lyophilized
Quantity 8 x 200 µl + 1 x 50 µg (Lhcb1) + 1 x 50 µl (Lhcb6)
Reconstitution
Storage

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications Western Blot (WB)
Related products

Other antibodies to pigment-binding proteins

Recommended secondary antibody

Plant protein extraction buffer

Secondary antibodies

Additional information

This set contains the following antibodies:

Product number:

Product name:

AS01 005
AS01 006
AS01 007
AS01 008
AS01 004
AS01 003
AS01 002
AS04 045
AS01 009
AS01 010

Anti-Lhca1
Anti-Lhca2
Anti-Lhca3
Anti-Lhca4
Anti-Lhcb
Anti-Lhcb2
Anti-Lhcb3
Anti-Lhcb4
Anti-Lhcb5
Anti-Lhcb6

application information
Recommended dilution

1:2000 - 1:10 000 with standard ECL (WB)

Expected | apparent MW

20 - 29 kDa

Confirmed reactivity
Higher plants including A.thaliana, H.vulgare, P.abies, S.oleracea, Drosera capensis

These antibodies have been shown to be reactive in all dicots, monocots, and gymnosperms tested so far; some of them have even been found to be reactive against Lhc-proteins of Chlamydomonas reinhardtii
Predicted reactivity
Not reactive in

No confirmed exceptions from predicted reactivity known in the moment

Additional information
Selected references Yoshida et al. (2016). Hisabori T1.Two distinct redox cascades cooperatively regulate chloroplast functions and sustain plant viability. Proc Natl Acad Sci U S A. 2016 Jul 5;113(27):E3967-76. doi: 10.1073/pnas.1604101113. Epub 2016 Jun 22.
Pavlovič et al. (2016). A carnivorous sundew plant prefers protein over chitin as a source of nitrogen from its traps. Plant Physiol Biochem. 2016 Mar 5;104:11-16. doi: 10.1016/j.plaphy.2016.03.008
Xu et al. (2011). Light-harvesting chlorophyll a/b-binding proteins are required for stomatal response to abscisic acid in Arabidopsis. J. Ex. Bot. Dec 5 (ahead of print).
Kang et al. (2010). Evaluation of light-harvesting complex proteins as senescence-related protein markers in detached rice leaves. Photosynthetica 47, 4:638-640.


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