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APC | allophycocyanin alpha and beta

345 €

AS08 277  |  clonality:polyclonal  |  host:rabbit  |  reactivity: red algae, cyanobacteria

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AS08 277

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product information
Background

Allophycocyanin is a protein of the light-harvesting phycobiliprotein family of red algae and cyanobacteria. It is an accessory pigment to chlorophyll A located in the core of the phycobilisome, and its strong spectral overlap with chlorophyll facilitates energy transfer for photosynthesis.

Immunogen

native allophycocyanin alpha and beta purified from Porphyridium cruentum phycobilisomes

Host Rabbit
Clonality Polyclonal
Clone
Purity Serum
Format Lyophilized
Quantity 200 ĩl
Reconstitution For reconstitution add 200 ĩl of sterile water.
Storage

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications Western Blot (WB), Immunolocalization (IL)
Related products

AS08 278 | anti-R-PC | R-phycocyanin

AS08 279 | anti-b-PE | phycoerythrobilin

AS08 280 | anti-B-PE | phycoerythrobilin and phycourobilin

Additional information
application information
Recommended dilution

1:1500 - 3000 (WB), 1: 200 - 1: 500  (IL)

Expected | apparent MW

14-19 kDa

Confirmed reactivity Porphyridium cruentum, Synechocystis PCC 6803
Predicted reactivity

red algae, cyanobacteria

Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information
Selected references Gandini et al. (2017). The transporter SynPAM71 is located in the plasma membrane and thylakoids, and mediates manganese tolerance in Synechocystis PCC6803. New Phytol. 2017 Mar 20. doi: 10.1111/nph.14526.
Gunnelius et al. (2014). The omega subunit of the RNA polymerase core directs transcription efficiency in cyanobacteria. Nucleic Acids Res. 2014 Jan 29.
Hernandez-Prieto et al. (2011). The small CAB-like proteins of the cyanobacterium Synechocystis sp. PCC 6803: Their involvement in chlorophyll biogenesis for Photosystem II. Bioch.Bioph. Acta.
Gantt & Lipschultz (1974). Phycobilisome structure by immuno-electron microscopy. J. Phycology, Vol. 13:3, pages: 185-192. (immunolocalization)
Gantt & Lipschultz (1974). Phycobilisomes of Porphyridium cruentum: Pigment Analysis. Biochem. 13:2960. Gantt E & C Lipschultz (1977). Probing phycobilisome structure by immuno-electron microscopy. J Phycol. 13:18


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