HliD | High light inducible protein
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|Recommended dilution||1: 2000 with standard ECL (WB)|
|Expected | apparent MW||
|Confirmed reactivity||Synechocystis sp. PCC 6803|
|Predicted reactivity||According to sequence analysis antibody may react with homologous Hli protein(-s) from Anabaena, Thermosynechococcus, Gloeobacter, Prochlorococcus, Synechococcus and Crocosphaera.|
|Not reactive in||no confirmed exceptions from predicted reactivity known in the moment|
|Additional information||to be added when available|
|Selected references||Staleva et al. (2015). Mechanism of photoprotection in the cyanobacterial ancestor of plant antenna proteins. Nat Chem Biol. 2015 Apr;11(4):287-91. doi: 10.1038/nchembio.1755.
Chidgey et al. (2014). A cyanobacterial chlorophyll synthase-HliD complex associates with the Ycf39 protein and the YidC/Alb3 insertase. Plant Cell. 2014 Mar;26(3):1267-79. doi: 10.1105/tpc.114.124495.
Knoppová et al. (2014). Discovery of a chlorophyll binding protein complex involved in the early steps of photosystem II assembly in Synechocystis. Plant Cell. 2014 Mar;26(3):1200-12. doi: 10.1105/tpc.114.123919. Epub 2014 Mar 28.
40 µg of membrane proteins from Synechocystis 6803 WT and DhliD strains was separated on 12-20 % SDS-PAGE and blotted 3h to PVDF. Blot was blocked with 0.2% Tween 20 in TBS for 1h at room temperature with agitation and than incubated in the primary antibody at a dilution of 1: 2 000 overnight at 10°C with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed twice for 5 min in TBS-T at room temperature with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1: 10 000 in for 1h at room temperature with agitation. Finally, the blot was washed as above, developed for 5 min with ECL (Plus Supersignal West Pico) according to the manufacturers instructions and exposed for 30 min in LAS 4000 imager.
WT and ΔScpE deletion mutants grown at 40 μE m-2s-1 and exposed to HL 2000 μE m-2s-1 for 30 min.
Courtesy of Dr. Josef Komenda, Academy of Science of Czech Republic
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