Lhca2 | PSI type II chloropyll a/b-binding protein

323 €

AS01 006  |  clonality: polyclonal  |  host: rabbit  |  reactivity: monocots and dicots; A. thaliana, A. hypogaea, B. corticulans, C. quitensis Kunt Bartl, C.reinhardti (one Lhca-type), H. vulgare, N. tabacum, O. sativa, P. sativum, P. vulgaris, P. patens, P. banksiana, Prasinoderma sp., Pyramimonas sp., S. oleracea, Z. mays


38 st
Item No:
AS01 006

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product information

The light-harvesting protein Lhca2 is one of the four main and highly conserved types of chlorophyll a/b-binding proteins (Lhca1-4) of the light harvesting antenna (LHCI) of plant photosystem I. Lhca2 is imported as a precursor from the cytosol into the chloroplast. Upon integration in the thylakoid membrane Lhca2 forms a heterodimer (LHCI-680) with Lhca3 that associates with the PSI core close to PsaF and PsaK.
A biochemical characterization of the plant LHCI antenna can be found in Klimmek et al. (2005) The structure of the higher plant light harvesting complex I: in vivo characterization and structural interdependence of the Lhca proteins. Biochemistry 44: 3065–3073.


BSA-conjugated synthetic peptide derived from the Lhca2 protein of Arabidopsis thaliana UniProt: Q9SYW8, Q8LCQ4, TAIR: At3g61470. This sequence is highly conserved in Lhca2 proteins of angiosperms (monocots and dicots) and gymnosperms as well as in At1g19150. This gene codes for the very low expressed Lhca6 protein which also has been denoted as Lhca2*1.

Host Rabbit
Clonality Polyclonal
Purity Total IgG
Format Lyophilized in PBS pH 7.4.
Quantity 200 µl
Reconstitution For reconstitution add 200 µl of sterile water.

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications Western Blot (WB)
Related products

AS01 011 | A set of 10 plant anti-Lhca and anti-Lhcb antibodies

AS01 011 Chlamydomonas | A set of anti-Lhc antibodies for Chlamydmonas

Available antibodies against pigment-binding proteins- LHC

recommended secondary antibody

Plant protein extraction buffer

Secondary antibodies

Additional information
Antibody format is a total IgG fraction, which means that it is a pool of polyclonal antibodies obtained by purification of serum on Protein G, not on a specific antigen column.
application information
Recommended dilution

1 : 2000 - 1: 5000 with ECL (WB)

Expected | apparent MW

27.7 | 24 kDa for Arabidopsis thaliana

Confirmed reactivity

Arabidopsis thaliana, Arachis hypogaea, Bryopsis corticulans, Colobanthus quitensis Kunt Bartl, Chlamydomonas reinhardti (one Lhca-type), Cytisus cantabricus (Wilk.) Rchb. F., Hieracium pilosella L, Hordeum vulgare, Lasallia hispanica, Nicotiana tabacum, Oryza sativa, Pisum sativum, Phaseolus vulgaris, Physcomitrella patens, Pinus banksiana (the higher of the two bands detected at 24 and 30 kDa is not considered to be specific to any Lhc protein), Prasinoderma sp., Pyramimonas sp. Spinacia oleracea, Syntrichia muralis (Hedw.) Raab, Zea mays

Predicted reactivity

angiosperms (monocots and dicots), gymnosperms

Not reactive in

No confirmed exceptions from predicted reactivity known in the moment

Additional information
Selected references Míguez et al. (2017). Diversity of winter photoinhibitory responses: A case study in co-occurring lichens, mosses, herbs and woody plants from subalpine environments. Physiol Plant. 2017 Feb 14. doi: 10.1111/ppl.12551.
Hu et al. (2017). The SUFBC2 D Complex is Required for the Biogenesis of All Major Classes of Plastid Fe-S Proteins. Plant J. 2017 Jan 19. doi: 10.1111/tpj.13483.
Kunugi et al. (2016). Evolution of Green Plants Accompanied Changes in Light-Harvesting Systems. Plant Cell Physiol. 2016 Jun;57(6):1231-43. doi: 10.1093/pcp/pcw071. Epub 2016 Apr 6.
Qin et al. (2014). Isolation and characterization of a PSI-LHCI super-complex and its sub-complexes from a siphonaceous marine green alga, Bryopsis Corticulans. Photosynth Res. 2014 Sep 12.

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