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Lhcb4 | CP29 chlorophyll a/b binding protein of plant PSII

323 €

AS04 045  |  clonality: polyclonal  |  host: rabbit  |  reactivity: A. thaliana, C. sativus, H. vulgare, N. tabacum, O. sativa, P. sativum, P. vulgaris, T. aestivum,  Z. mays

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AS04 045

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product information
Background

Lhcb4 (CP29) is one of the 3 minor chlrorophyll a/b-binding proteins associated with Photosystem II in plants and algae. Lhcb4 has been suggested to act in the regulation of the chl a excited state concentration of PSII because of its ability of sensing lumenal pH resulting in reversible phosphorylation. In Arabidopsis thaliana 2 genes code for two isoforms Lhcb4.1 and Lhcb4.2. A third isoform (Lhcb4.3, At2g40100), probably only present in dicots, has found to be differently regulated and therefore has been denoted as Lhcb8.

Immunogen

BSA-conjugated synthetic peptide derived from a highly conserved sequence of Lhb4 proteins from angiosperms (monocots and dicots) and gymnosperms, including Arabidopsis thaliana (Lhcb4.1 At5g01530 and Lhcb4.2 At3g08940 and Lhcb4.3 At2G40100).

Host Rabbit
Clonality Polyclonal
Clone
Purity Total IgG
Format Lyophilized in PBS pH 7.4
Quantity 200 µl
Reconstitution For reconstitution add 200 µl of sterile water.
Storage

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications Western Blot (WB)
Related products

AS06 117 Lhcb4 | CP29 (Lhcb4) homolog, Chlamydomonas

LHC  available antibodies against pigment-binding proteins

PSII  available antibodies against Photosystem II proteins

AS04 045PRE Lhcb4 | CP29 chlorophyll a/b binding protein of plant PSII, pre-immune serum

Plant protein extraction buffer

Secondary antibodies

Additional information

An overview about the different Lhc-protein types in plants can be found in Klimmek et al. (2006) Abundantly and rarely expressed Lhc protein genes exhibit distinct regulation patterns in plants. Plant Physiol 140: 793-804.

application information
Recommended dilution

1:7 000 (WB)

Expected | apparent MW

31.9 | 29 kDa for Arabidopsis thaliana

Confirmed reactivity

Arabidopsis thaliana, Cucumis sativus L. cv. Jihong no. 2, Drosera capensis, Hordeum vulgare, Nicotiana tabacum, Oryza sativa, Pisum sativum, Phaseolus vulgaris, Triticum aestivum, Zea mays

Predicted reactivity

Cucumis sativus, Populus, gymnosperms and microalgae Ostrecococcus tauri; the target sequence is only weakly conserved in Physcomitrella patens

Not reactive in

Chlamydomonas reinhardtii (please use AS06 117 for this organism)

Additional information

For image of detection please refer to Kusaba et al. (2007).

Protein is processed into mature form (Jansson 1999).

Selected references Tyuereva et al. (2017). The absence of chlorophyll b affects lateral mobility of photosynthetic complexes and lipids in grana membranes of Arabidopsis and barley chlorina mutants. Photosynth Res. 2017 Apr 5. doi: 10.1007/s11120-017-0376-9. (Hordeum vulgare, western blot)
Yang-Er Chen et al. (2017). Responses of photosystem II and antioxidative systems to high light and high temperature co-stress in wheat. J. of Exp. Botany, Volume 135, March 2017, Pages 45–55.
Betterle et al. (2016). The STN8 kinase-PBCP phosphatase system is responsible for high-light-induced reversible phosphorylation of the PSII inner antenna subunit CP29 in rice. Plant J. 2016 Nov 4. doi: 10.1111/tpj.13412. [Epub ahead of print]
Pavlovič et al. (2016). A carnivorous sundew plant prefers protein over chitin as a source of nitrogen from its traps. Plant Physiol Biochem. 2016 Mar 5;104:11-16. doi: 10.1016/j.plaphy.2016.03.008
Kim et al. (2015). Cytosolic targeting factor AKR2A captures chloroplast outer membrane-localized client proteins at the ribosome during translation. Nat Commun. 2015 Apr 16;6:6843. doi: 10.1038/ncomms7843.
Sun et al. (2014). Direct energy transfer from the major antenna to the photosystem II core complexes in the absence of minor antennae in liposomes. Biochim Biophys Acta. 2014 Nov 22. pii: S0005-2728(14)00650-1. doi: 10.1016/j.bbabio.2014.11.005.
Grimmer et al. (2014). The RNA-binding protein RNP29 is an unusual Toc159 transport substrate. Front. Plant Sci. | doi: 10.3389/fpls.2014.00258

Application example

Western blot using anti-Lhcb4 antibodies

5 µg of total protein from embebed seeds of Nicotiana tabacum growing during 4 d in dark (0) and then transfer to continue light growing for 6 h (6) extracted with LB2x buffer and denatured 90 ºC for 2-5 min, were separated on 12.5 % SDS-PAGE and blotted 1h to PVDF using tank transfer. Blots were blocked with TBS-T with 5% dry-milk for 3h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 10 000 overnight at 4 ºC with agitation in TBS-T with 5% dry-milk. The antibody solution was decanted and the blot was rinsed briefly twice, then washed 4 times for 15 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera) diluted to 1:30 000 in TBS-T with 5% dry-milk for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL Plus western blotting detection system by GE Healthcare. Exposure time was 60 seconds.

Courtesy of Dr. Concha Almoguera, Inst. de Recursos Naturales y Agrobiología –CSIC, Spain

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