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Lhcbm5 | Chlorophyll a-b binding protein of LHCII

265 €
Buy 2 items of this product for 198 €/each
Buy 3 items of this product for 180 €/each

AS09 408 | clonality:polyclonal | host:rabbit | reactivity: Chlamydomonas reinhardtii

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AS09 408

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product information
Background

Lhcbm5 is one of minor LHCII that associates with PSI in state-2

Immunogen

SDS-PAGE purified polypeptide from Chlamydomonas reinhardtii LHCII-type II-enriched fractions

Host Rabbit
Clonality Polyclonal
Clone
Purity Serum
Format Lyophilized
Quantity 50 ĩl
Reconstitution For reconstitution add 50 ĩl of sterile water
Storage

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications Western blot (WB)
Related products

AS06 117 | anti-Lhcb4 | CP29 (Lhcb4) rabbit antibody

AS09 407 | anti-Lhcb5 CP26 rabbit antibody

Collection of antibodies to Chlamydomonas proteins

Plant and algal protein extraction buffer

Secondary antibodies

Additional information

This antibody cross-reacts with three major LHCII proteins of Chlamydomonas, which are slightly smaller than Lhcam5 on SDS-gel. 6M urea SDS-PAGE is one of the best systems that separate Lhcbm5 and the other major LHCII proteins. The dilution of the antibody should be carefully determined to reduce the cross-reactions with other major LHCII proteins, we recommend for this purpose to use the dilution of 1: 10 000- 1: 50 000

application information
Recommended dilution

1: 5000 - 1: 10 000 with ECL (WB)

Expected | apparent MW

29 | 30 kDa

Confirmed reactivity

Chlamydomonas reinhardtii

Predicted reactivity

Chlamydomonas reinhardtii

Not reactive in

n.a.

Additional information

for western blot detection image please refer to the article below

Selected references Jeong et al. (2016). Loss of CpSRP54 function leads to a truncated light-harvesting antenna size in Chlamydomonas reinhardtii. Biochim Biophys Acta. 2016 Oct 17;1858(1):45-55. doi: 10.1016/j.bbabio.2016.10.007.
Grewe et al. (2014). Light-Harvesting Complex Protein LHCBM9 Is Critical for Photosystem II Activity and Hydrogen Production in Chlamydomonas reinhardtii. Plant Cell. 2014 Apr 4;26(4):1598-1611.


||| For other applications, usage on species other than stated above or any other questions, please use the LiveChat option or contact us at support@agrisera.com