AS14 2819 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Chlamydomonas reinhardtii
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1: 1000 (WB)
|Expected | apparent MW||
|Confirmed reactivity||Chlamydomonas reinhardtii|
|Not reactive in||Phaeodactylum tricornutum|
|Selected references||Allorent et al. (2016). UV-B photoreceptor-mediated protection of the photosynthetic machinery in Chlamydomonas reinhardtii. Proc Natl Acad Sci U S A. 2016 Dec 20;113(51):14864-14869. doi: 10.1073/pnas.1607695114. Epub 2016 Dec 5.
Dinc et al. (2016). LHCSR1 induces a fast and reversible pH-dependent fluorescence quenching in LHCII in Chlamydomonas reinhardtii cells. Proc Natl Acad Sci U S A. 2016 Jul 5;113(27):7673-8. doi: 10.1073/pnas.1605380113. Epub 2016 Jun 22.
Correa-Galvis et al. (2016). Photosystem II Subunit PsbS Is Involved in the Induction of LHCSR Protein-dependent Energy Dissipation in Chlamydomonas reinhardtii. J Biol Chem. 2016 Aug 12;291(33):17478-87. doi: 10.1074/jbc.M116.737312.
10 ug of a total cell extract of Chlamydomonas reinhardtii: WT _P _HL_High CO2 (1), WT_P_HL (2), Npq4_P _HL (3), were separated on Bolt® 4-12% Bis-Tris Plus Gels (precast) and blotted to PVDF using Bolt® transfer system for 1h . Blots were blocked with 5% BSA/milk for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and
Courtesy Dr. Roberta Croce, Biophysics of Photosynthesis Dep. Physics and Astronomy Faculty of Sciences VU University Amsterdam, The Netherlands
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