ANTIBODY SHOP

Services
Name:
Phone:
E-mail:
Message

PrxQ | Peroxiredoxin, thioredoxin reductase

345 €

AS05 093 | clonality: polyclonal | host:: rabbit | reactivity: A. thaliana, M. esculenta, S. oleracea, Z. mays

PRODUCT INFORMATION IN PDF

Qty: 
Availability:
46 st
Delivery:
Shipping:
Item No:
AS05 093

Info: Product suggestions Read reviews
product information
Background

Peroxiredoxins (EC=1.11.1.15) belong to the enzyme family which is ubiquitous in all kingdoms of life. Prx Q enzyme acting by reducing hydroperoxides. Peroxiredoxins have no heme group, unlike the other peroxidases, but perform their enzymatic activity using cysteine residues with redox-active thiol groups. The ability of peroxiredoxins to hydrolyze hydroperoxides suggests that this protein family has a general function in oxidant defence.

Immunogen

His-tagged full length protein (with presequence) of Arabidopsis thaliana was overexpressed in in E.coli. Isolated with HiTrap column (GE Healthcare) Q9LU86, At3g26060

Host Rabbit
Clonality Polyclonal
Clone
Purity Serum
Format Lyophilized
Quantity 200 µl
Reconstitution For reconstitution add 200 µl of sterile water.
Storage

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications Western Blot (WB)
Related products

Plant protein extraction buffer

Secondary antibodies

Additional information

This product can be sold containing proclin if requested.

application information
Recommended dilution

1: 5000 with standard ECL (WB)

Expected | apparent MW

16 kDA

Confirmed reactivity Arabidopsis thaliana, Manihot esculenta, Spinacia oleracea, Zea mays
Predicted reactivity

Populus spp. , monocots including Triticum aestivum, Oryza sativa

Not reactive in

No confirmed exceptions from predicted reactivity known in the moment

Additional information

In stroma fractions a weak background reaction at 28 kDa is visible. No crossreactivity in any thylakoid fractions.

Selected references Yoshida et al. (2016). Hisabori T1.Two distinct redox cascades cooperatively regulate chloroplast functions and sustain plant viability. Proc Natl Acad Sci U S A. 2016 Jul 5;113(27):E3967-76. doi: 10.1073/pnas.1604101113. Epub 2016 Jun 22.
Yoshida et al. (2015). Thioredoxin Selectivity for Thiol-Based Redox Regulation of Target Proteins in Chloroplasts. J Biol Chem. 2015 Apr 15. pii: jbc.M115.647545.
Feifei et al. (2014). Comparison of Leaf Proteomes of Cassava (Manihot esculenta Crantz) Cultivar NZ199 Diploid and Autotetraploid Genotypes. PLoS One. 2014 Apr 11;9(4):e85991. doi: 10.1371/journal.pone.0085991. eCollection 2014.
Wu et al. (2013). Proteomic and Phytohormone Analysis of the Response of Maize (Zea mays L.) Seedlings to Sugarcane Mosaic Virus. PLoS One. July 23;8(7).


||| For other applications, usage on species other than stated above or any other questions, please use the LiveChat option or contact us at support@agrisera.com