SVR4-like | Supressor of variegation 4 - like

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AS13 2726   | clonality: polyclonal  |  host: rabbit  |  reactivity: Arabidopsis thaliana, Hordeum vulgare



26 st
Item No:
AS13 2726

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product information

SVR4-like (Supressor of variegation 4-like) is a homolog of SVR4. It is a nuclear-encoded chloroplast-located protein required for proper function of the plastid transcriptional machinery. Synonymes: MRL7-like, MESOPHYLL-CELL RNAI LIBRARY LINE 7-LIKE, MRL7-L.


KLH-conjugated synthetic peptide derived from Arabidopsis thaliana SVR4-like protein sequence, UniProt: Q84RF5, TAIR: At2g31840

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 ĩl
Reconstitution For reconstitution add 50 ĩl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Related products

AS13 2725 | SVR4 | Supressor of variegation 4, rabbit antibodies

Plant protein extraction buffer

Secondary antibodies

Additional information

This protein is present in very low amounts only in early stages of plant development and this has to be taken into account when harvesting the tissue.

Western blots were done on: Arabidopsis thaliana total protein extract from cotyledons and Hordeum vulgare (2-14 days old plants).

application information
Recommended dilution 1 : 1000 (WB)
Expected | apparent MW

40 | 42 kDa

Confirmed reactivity Arabidopsis thaliana, Horderum vulgare
Predicted reactivity
Not reactive in No confirmed exceptions from predicted reactivity are currently known.
Additional information
Selected references Powikrowska et al. (2013). SVR4 of variegation 4 and SVR4-like two proteins with a role in proper organization of the chloroplast genetic machinery. Physiol Plant. Sep 23. doi: 10.1111/ppl.12108.

application example

western blot using anti-SVR4-like antibodies

SVR4-like antibody tested on intact chloroplasts isolated from ome week old barley. Intact chloroplasts were isolated from 1 week old  Hordeum vulgare plants. The sample corresponding to 4ug Chl and was separated on 12% Criterion XT Bis-Tris SDS-PAGE (BioRad) gels and blotted for 25min 100V to PVDF membrane. Blot was blocked with 5% fat free skimmed milk in PBS-T for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1000 overnight with agitation in 4˚C. The antibody solution was decanted and the blot was washed 3 times for 5 min in PBS-T at RT with agitation. Blot was incubated in secondary antibody (swine anti-rabbit IgG horse radish peroxidase conjugated, from Dako) diluted to 1: 5000 in 1% fat free skimmed milk in PBS-T for 1h at RT with agitation. The blot was washed 5 min in PBS-T and 1 min in PBS developed for 5 min with ECL according to the manufacturers instructions. Exposure time was 20 min.

Courtesy of Dr. Małgorzata Powikrowska, University of Copenhagen, Danmark

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