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Tlp18.3 | Thylakoid lumen 18.3 kDa protein

345 €

AS08 369  |  clonality: polyclonal  |  host: rabbit  |  reactivity: A. thaliana, Z.mays

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Item No:
AS08 369

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product information
Background

UPF0603 protein At1g54780, chloroplastic is located in thylakoid lumen. The protein seem to be required for high light tolerance of PSII. Sirpiö et al. (2007) TLP18.3, a novel thylakoid lumen protein regulating photosystem II repair cycle. Biochem J; 406(3): 415-425

Immunogen

synthetic peptides amino acids 227-241 and amino acids 242-256 from Arabidopsis thaliana Tlp18.3 protein sequence (accession At1g54780)

Host Rabbit
Clonality Polyclonal
Clone
Purity Serum
Format Lyophilized
Quantity 200 ĩl
Reconstitution For reconstitution add 200 ĩl of sterile water
Storage

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications Western blot (WB)
Related products
Additional information
application information
Recommended dilution

1: 1000 with standard ECL (WB)

Expected | apparent MW

22 | 18.5 kDa for A. thaliana

Confirmed reactivity

Arabidopsis thaliana

Predicted reactivity

Oryza sativa, Ricinus communis, Zea mays

Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information

application note: in a gel without urea Tlp18.3 will run at 18 kDa, while in a gel with urea it runs at 25 kDa

Selected references

Zienkiewicz et al. (2013).Light intensity and quality stimulated Deg1-dependent cleavage of PSII components in the chloroplasts of maize. Plan Physiol Biochem. March 16.


application example

 

4, 2, and 1μg chl/lane (from left to right) of chloroplast prepared from Arabidopsis thaliana were separated on a 15% gel with 6M urea and blotted to PVDF. Filters were blocked (1h) with 5% milk powder and incubated with anti-Tlp18.3 antibody (1: 1000, 1h) followed by incubation with secondary HRP-coupled anti-rabbit antibody (1: 10 000, 1h). Signal was detected with standard ECL.
AS08 369 western blot.jpg

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