Tlp18.3 | Thylakoid lumen 18.3 kDa protein
AS08 369 | clonality: polyclonal | host: rabbit | reactivity: A. thaliana, Z.mays
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1: 1000 with standard ECL (WB)
|Expected | apparent MW||
22 | 18.5 kDa for A. thaliana
Oryza sativa, Ricinus communis, Zea mays
|Not reactive in||
no confirmed exceptions from predicted reactivity known in the moment
application note: in a gel without urea Tlp18.3 will run at 18 kDa, while in a gel with urea it runs at 25 kDa
Zienkiewicz et al. (2013).Light intensity and quality stimulated Deg1-dependent cleavage of PSII components in the chloroplasts of maize. Plan Physiol Biochem. March 16.
4, 2, and 1μg chl/lane (from left to right) of chloroplast prepared from Arabidopsis thaliana were separated on a 15% gel with 6M urea and blotted to PVDF. Filters were blocked (1h) with 5% milk powder and incubated with anti-Tlp18.3 antibody (1: 1000, 1h) followed by incubation with secondary HRP-coupled anti-rabbit antibody (1: 10 000, 1h). Signal was detected with standard ECL.
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