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FtsH6 | ATP-dependent zinc metalloprotease FtsH6 (chloroplastic)

345 €

AS05 094A  |  clonality: polyclonal  | host: rabbit  |  reactivity: Arabidopsis thaliana

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AS05 094A

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Background

Degradation of the most abundant membrane protein on earth, the light-harvesting complex of Photosystem II (LHC II), is highly regulated under various environmental conditions, e.g., light stress, to prevent photochemical damage to the reaction center. FtsH6 is proposed to be a general LHC II protease and FtsH6-dependent LHC II proteolysis can be  a feature of all higher plants.

Immunogen

KLH-conjugated  peptide chosen from AtFtsH6 protein sequence of Arabidopsis thaliana  Q1PDW5

Host Rabbit
Clonality Polyclonal
Clone
Purity Affinity purified serum
Format Lyophilized in PBS pH 7.4
Quantity 2x100 ĩg
Reconstitution For reconstitution add 91 ĩl of sterile water.
Storage

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications western blot (WB)
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AS16 3930 | anti-FtsH1 + FtsH5 | ATP-dependent zinc metalloprotease FtsH1 + FtsH5 (chloroplastic)
AS16 3929 | anti-FtsH2 + FtsH8 | ATP-dependent zinc metalloprotease FtsH2 + FtsH8 (chloroplastic)
AS07 204 | anti-FtsH3 + FtsH10 | ATP-dependent zinc metalloprotease FtsH3 + FtsH10 (mitochondrial)
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Antibodies to other proteins involved in photosynthesis

Plant and algal protein extraction buffer

Secondary antibodies

Additional information
application information
Recommended dilution

1: 1 500 - 1: 1000 with standard ECL (WB)

Expected | apparent MW

74.36 kDa

Confirmed reactivity

Arabidopsis thaliana

Predicted reactivity

Arabidopsis thaliana

Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information

to be added when available

Selected references
Sedaghatmehr et al. (2016). The plastid metalloprotease FtsH6 and small heat shock protein HSP21 jointly regulate thermomemory in Arabidopsis. Nat Commun. 2016 Aug 26;7:12439. doi: 10.1038/ncomms12439.


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