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Deg1 | chloroplastic DegP-type serine protease 1

345 €

AS07 246 | clonality: polyclonal | host: rabbit | reactivity: A. thaliana, P. sativum, Z. mays

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AS07 246

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product information
Background

Deg1 belongs to S1 chymotrypsin protease family and contains 16 protein members in Arabidopsis thaliana. Deg1 is localized to chloroplast.

Immunogen

Recombinant Deg1 of Arabidopsis thaliana O22609, At3g27925, overexpressed in E.coli

Host Rabbit
Clonality Polyclonal
Clone
Purity Total IgG
Format Lyophilized in PBS pH 7.4
Quantity 360 ĩg
Reconstitution For reconstitution add 200 ĩl of sterile water.
Storage

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications western blot (WB)
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AS14 2767 | Deg8 | protease Do-like 8, chloroplastic, rabbit antibody
AS10 711 | DEG15 | endopeptidase, peroxisomal marker, rabbit antibody
collection of antibodies to chloroplastic proteases

Plant protein extraction buffer

Secondary antibodies

Additional information
application information
Recommended dilution

1: 500 on 5 ug thylakoid fraction (WB)

Expected | apparent MW

46.6 | 36 kDa

Confirmed reactivity

Arabidopsis thaliana, Pisum sativum, Zea mays

Predicted reactivity

dicots including: Ricinus communis

Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information

unspecific reaction at ca. 30 kDa and ca. 85 kDa (checked by by mass spec, and confirmed not to be neither Deg1 nor any other protease. The band at the right MW does contain Deg1 by our MS analysis

Selected references Zienkiewicz et al. (2013). Light intensity and quality stimulated Deg1-dependent cleavage of PSII components in the chloroplasts of maize. Plant Physiol. & Biochem. March 16.

application example

 

(1) Pisum sativum thylakoid fraction, (2) Arabidopsis thaliana thylakoid fraction, (3) recombinant Deg1 expressed in E.coli. Primary antibody has been used at 1: 500 dilution with standard ECL.

Western blot using anti-Deg1 antibodies


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