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HSP70 | Positive control/quantitation standard

169 €
Buy 2 items of this product for 140 €/each
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AS08 371S  |  protein standard for quantitation and positive control

PRODUCT INFORMATION IN PDF

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AS08 371S

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product information
Background

Heat-shock protein 70 (Hsp70) is the major stress-inducible protein in vertebrates and is highly conserved throughout evolution. It plays a role as a molecular chaperone and is important for allowing cells to cope with acute stressor insult, especially those affecting the protein machinery. Heat shock cognate protein 70 (HSC70), is a highly conserved protein and a member of the family of molecular chaperones.

Source of HSP70 standard: recombinant Hsp70 of Arabidopsis thaliana UniProt: Q9LHA8, TAIR: AT3G12580

Host
Clonality
Clone
Purity
Format
Quantity
Reconstitution For reconstitution add 82 ĩl of milliQ water. Please notice that this product contains glycerol and might appear as liquid but is provided lyophilized.

Final standard concentration is going to be 0.15 pmol/ĩl.
Storage

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications western blot (WB)
Related products

Primary antibody matching HSP70 standard are:

AS08 371 | anti-HSP70 | heat shock protein 70 cytoplasmic, rabbit antibody

collection of other protein standards

collection of matching global antibodies

Plant and algal protein extraction buffer

Additional information

The HSP70 protein standard can be used in a combination with Agrisera global antibiodies (AS08 371) to quantitate HSP70 from a wide range of species. Global antibodies are raised against highly conserved amino acid sequence. 

Quantitative western blot:  detailed method description, video tutorial

application information
Recommended dilution

Standard curve: 3 loads are recommended (eg 0.1, 0.2, 0.3 pmol). Adjust range to fit your samples and your experiment.

For most applications a sample load of 0.2 μg of chlorophyll/well will give a HSP70 signal in this range.

Positive control: a 2 μl load per well is optimal for most chemiluminescent detection systems. Higher standard concentration needs to be used to allow detection by Coomasie stains. Such gels with higher standard concentration can not be used for quantitation using chemiluminescence.

This standard is stabilized and ready and does not require heating before loading on the gel.

Please note that this product contains 10% glycerol and might appear as liquid but is provided lyophilized. Allow the product several minutes to solubilize after adding water. Mix thoroughly but gently Take extra care to mix thoroughly before each use, as the proteins tend to settle with the more dense layer after freezing.

Expected | apparent MW

70 kDa

Confirmed reactivity
Predicted reactivity
Not reactive in
Additional information

Concentration: after re-constitution with sterile milliQ water final concentration of the standard is 0.15 pmoles/µl

Protein standard buffer composition: Glycerol 10%, Tris Base 141 mM, Tris HCl 106 mM, LDS 2%, EDTA 0.51 mM, SERVA® Blue G250 0.22 mM, Phenol Red 0.175 mM, pH 8.5, 0.1mg/ml PefaBloc protease inhibitor (Roche), 50 mM DTT.

This standard is ready-to-load and does not require any additions or heating. It needs to be fully thawed and thoroughly mixed prior to using. Avoid vigorous vortexing, as buffers contain detergent. Following mixing, briefly pulse in a microcentrifuge to collect material from cap.
This standard is stabilized and ready and does not require heating before loading on the gel.
Please note that this product contains 10% glycerol and might appear as liquid but is provided lyophilized. Allow the product several minutes to solubilize after adding water. Mix thoroughly but gently Take extra care to mix thoroughly before each use, as the proteins tend to settle with the more dense layer after freezing.

Selected references to be added when available, product released in September 2015

application example


Quantitation:
When quantitated standards are included on the blot, the samples can be quantitated using the available software. Excellent quantitation can be obtained with images captured on the Bio-Rad Fluor-S-Max or equivalent instrument using Bio-Rad QuantityOne software. The contour tool is used to the area for quantitation and the values are background subtracted to give an adjusted volume in counts for each standard and sample. Using above protocol linear standard curves are generated over 1-1.5 orders of magnitude range in target load. It is important to note that immunodetections usually show a strongly sigmoidal signal to load response curve, with a region of trace detection of low loads, a pseudolinear range and a region of saturated response with high loads. For immunoquantitation it is critical that the target proteins in the samples and the standard curve fall within the pseudolinear range. Our total detection range using this protocol spans over 2 orders of magnitude, but the quantifiable range is narrower.
Quantitative western blot:  detailed method description.


||| For other applications, usage on species other than stated above or any other questions, please use the LiveChat option or contact us at support@agrisera.com